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Cbx2 (NM_007623) Mouse Untagged Clone

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SKU
MC204062
Cbx2 (untagged) - Mouse chromobox homolog 2 (Drosophila Pc class) (Cbx2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $484.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cbx2
Synonyms M33; MOD2; pc
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC035199
GGCGGGGCGCGGCGGGGGCGGCGCTTTGTGTGCAGCAGTGAGCCGGGGTCTGCGGGGCGCGGGCCGCGGC GGGCGGCGGCATGGAGGAGCTGAGCAGCGTGGGCGAGCAGGTCTTCGCCGCCGAGTGCATCCTGAGCAAG CGGCTCCGCAAGGGCAAGCTGGAGTACCTGGTCAAGTGGCGCGGCTGGTCCTCCAAACACAACAGCTGGG AGCCAGAAGAGAACATTTTGGACCCGAGGCTGCTCCTAGCCTTCCAGAAGAAGGAACATGAGAAGGAGGT TCAGAACCGGAAGAGAGGCAAGAGACCCAGGGGCAGGCCGAGGAAACACACAGTCACATCCTCCTGCAGC CGGCGCTCCAAGCTCAAGGAACCAGATGCGCCATCCAAATCCAAATCCAGCAGTTCGTCCTCTTCCTCCA CATCTTCCTCCTCTTCCTCGGACGAAGAGGAAGACGACAGCGACCTAGACTCCAAGAGAGGCCCCCGGGG CCGTGAAACCCATCCAGTGCCTCAGAAAAAAGCCCAGATCCTGGTAGCCAAGCCAGAGCTGAAGGATCCC ATTAGAAAGAAACGGGGACGCAAGCCTCTACCCCCAGAACAGAAGGCAGCTCGGAGACCCGTCAGCCTGG CCAAGGTGCTAAAGACCACCAGGAAGGATCTGGGGACCTCAGCCGCCAAGCTGCCCCCTCCACTCAGCGC TCCGGTGGCAGGCCTGGCTGCCCTGAAGGCCCACACCAAAGAGGCCTGTGGTGGCCCCAGCACTATGGCG ACCCCAGAGAACCTGGCCAGTCTGATGAAAGGCATGGCCGGGAGCCCCAGCAGAGGCGGCATCTGGCAGA GCTCCATCGTACACTACATGAACCGCATGAGCCAGAGTCAGGTTCAGGCTGCCAGCCGACTGGCACTCAA GGCCCAGGCCACCAACAAGTGCGGTCTCGGGCTAGACCTGAAAGTGAGGACGCAGAAGGGGGGTGAGCTA GGGGGGAGCCCCGCAGGAGGCAAGGTCCCGAAGGCCCCCGGTGGCGGAGCTGCAGAGCAGCAGAGAGGGA ACCATTCGGGGAGCCCAGGTGCTCAGCTGGCACCCACTCAGGAGTTGAGCCTTCAGGTCCTTGACTTGCA AAGCGTCAAGAACGGTGTGCCTGGTGTGGGCCTGCTTGCTCGCCATGCCCCAGCCAAGGCTATTCCTGCT ACCAACCCAGCCACAGGGAAAGGTCCTGGGAGCGGCCCCACAGGAGCAAACATGACCAACGCTCCCACAG ACAACAACAAAGGGGAAAAGCTGACTTGCAAGGCAACGGCTCTGCCTGCCCCTTCCGTCAAGCGGGACAC CGTTAAAAGCGTCGCTGCCTCCGGCGGGCAGGAGGGCCACACAGCCCCGGGAGAAGGCCGAAAGCCACCT GCGCTGTCTGAGCTGAGCACGGGAGAGGAGAATAGTAGCTCTGACTCGGACCCTGACTCGACCTCGCTTC CCAGTGCTGCGCAGAACCTATCTGTAGCTATCCAGACCAGCCAGGACTGGAAACCTACCCGCAGTCTCAT CGAACACGTCTTTGTCACGGATGTCACAGCCAACCTCATCACTGTCACCGTGAAGGAGTCGCCCACCAGC GTGGGCTTCTTCAACTTGAGGCATTATTGAGACCATTGCCCGAGCCAGACCTGCCTTCCAGTCTCTGGTT TTGCTTTGATGCCTGACCTGGGGGCCAACTCTGCCCTGTCCTGAGACTCAGGGCTGGGAAATGGCTTCCT GCTGATTCTACCTCTCTGGGCCTCCACTTCAGTCTGTCCTGTCATCTCAGGAAAGCCGGGTGGCCACTGA CTTAAAAGCTACCTGCTTGGACATAGCATCCTTTCTACACATCCCTCCTTAACCTAGTGAGACCCTCCCC TCCCTGGCCAACTACTGTGAGGTGGATTGGGGAGGGGGGGGCACTGATACAGCTCCCTCCCCAGGCCGTT GAGGAGAGGGGTGGGGCTGGTAGGGCCTTCTCCCACATCCAGCCACGGCAGGGTCTTTCTGGTGAGGGAG ACACTCTATGCTTTGCAAGTAAGGCTATAACTTCAGTTTTAAAAGGCTAATTAGTTGTGTGGTTGGGCTC AGGGACCCTGCGGAAATCTTCAGTCTCTATCCGTGGTGTGGTGTCCCCTCTAGTTGCAATTGTAGTCCCA AAGCCCAGTCAGGGTTTGGAGTCCTGGCACTAAGGTGTACCTGGGTGCTTCTGGACTTGGGAATAGAGAG AGCCATGTCCTTATTGCCTCCTTCAAGCAGGCTCAAGCCCCAGTGAGAAGTGACCCTGTTCCTCTCTGGG TCTGGACCCATACCCCTGCTTTGGCCCACTAAATTGCACCCTAGTTCTGAGGTCTGACACTCCTGGGCTT GGGGAAGGAGCAGGCTAGACACCCAGAGCAAGCTGATGTAGGCACTTGGAGTCCTGGGCAAGCAATCTTC CTTCATGTTGGACTTTTATTACTAGGACCCCATAAGTCACCATGGTGGCACCAGTGTGGGAATGCAGGTG TTTTGTGGGGGCCTGTCACCCTGCTGCCTGTCTAGACATCTCTAAACTACTGTGGAAGCATTTTCTATAG TCAGTCTTGGTGCAGAGCTCCGCCTGGTGAGTGGGCCACTCTTGGGTCCCATATTCCCTGACAATCCTCT CCCACATTTGGGAGCTTGTGTTTCCTCCCTCGCTGAGAAGCAATAGCCCTATGCCATTTGTGTGGTGGGG TCGTTTAGGAGCCCACCTGGCAGGGGGCCCCTAATTTCTTTCCCATCCTAGGAGATAAGTCTAGTGCTGA GCCCCTTTCTAAATGCCTTCTCCCCTGTCCCCCATCCCGGGTAACTGTCTTGAAACTACCTTGTCAGATT GCTGGCCGATGACAGGATTTTCTGTCCTGGGATATCACGGTGGGCCGTGACAATCTGGTCAGTGAGTCTG TGATGCCAAAAAGTAGGGGTGCTATGAAACCCTGGTTCTGCTTCTGCCATTGACTGATCAGCCAGCAGGT GTGGGCTCTTCACTCCAGTTGCCTTGAAGGGAAATGTTTCCCCTTCTAAAACCAGTCAGACAGTGGGCTG AGGTGTCTGGGACCTGCCACACTTTCAAAGTGCACCCCACCCCCACCCCAGTAGCTCACCCTGCACACCA AATAAGATGGTCTTTGTGTAACCAAACCAACCTCAATGCTGTGCCTTTTGGGGCAGACCCATATCCACAG CTCCTGAGAGATGCCTGTGATGTGCCAGGGGACCATGTTACTGGCAGCAGGAGCTACGTACTGGGAAGAA TTCAGGGCCAGGAAGTGGGTAACAAAGGGGAAAGGGTTGCCTACTGCATTATCAATGTCCCTCCCTGCCC TCAGCCAGGGTCTGCAACCTTTGTACCAGACACTGCCTTTACAAACACCTCCCCGTCTCTTTGGGGTCCC ATGAGCTGTTGGAGAAGTGACCCTGTTTGGGGATCTGGGTTGACTGGCCTTTGTCCCCTGCTGCCAGTCT CCAAATCCCAATTTGGTGCCTTTTATTTTACTGGTTACGTCAGTCCCAAAGTTGAAATCTACAAATCCTT TCCTCTCAGCCACTTTGCCTTCTTGTGTGTTTTTCCTGGTGCTGTGACCCCAGTGGTCTATGTCACAAAG CACATGTGTGTAGTGCGCCAGCGCGAGCCCCACTGGAGCAGCGCCATTCGCTCTGAGTCAAGTTCTCGTG TTTACCGATAAATAAAGTAGTTGGTTTTTAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_007623
Insert Size 1560 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC035199, AAH35199
RefSeq Size 3754 bp
RefSeq ORF 1560 bp
Locus ID 12416
UniProt ID P30658
Cytogenetics 11 83.33 cM
Summary This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in results in male-to-female gonadal sex reversal. provided by RefSeq, Sep 2015
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"NM_007623" in other vectors (4)
SKU Description Size Price
MG208323 Cbx2 (tGFP-tagged) - Mouse chromobox homolog 2 (Drosophila Pc class) (Cbx2) 10 ug
$684.00
MR208323 Cbx2 (Myc-DDK-tagged) - Mouse chromobox homolog 2 (Drosophila Pc class) (Cbx2) 10 ug
$289.00 $484.00
MR208323L3 Lenti ORF clone of Cbx2 (Myc-DDK-tagged) - Mouse chromobox homolog 2 (Drosophila Pc class) (Cbx2) 10 ug
$784.00
MR208323L4 Lenti ORF clone of Cbx2 (mGFP-tagged) - Mouse chromobox homolog 2 (Drosophila Pc class) (Cbx2) 10 ug
$784.00

file-alt Citations

ZBTB32 is an early repressor of the CIITA and MHC class II gene expression during B cell differentiation to plasma cells
Yoon, Scharer, Majumder et al
J. Immunol. (2012) 189 (5), 2393-403 DOI: 10.4049/jimmunol.1103371
Cited by 85
AI Summary: OriGene products contributed to this study by providing expression plasmids for CBX2 (MC204062), HMGN3 (SC110044), and Rad54l (MC205395), as well as pLKO.1 lentiviral constructs containing shRNA targeting ZBTB32, which were used to investigate the regulatory role of ZBTB32 in CIITA and MHC class II gene expression during B cell differentiation.
Snippet: Expression plasmids used in transient transfection experiments, purchased from OriGene Technologies, included those for CBX2 (MC204062), HMGN3 (SC110044), Rad54l (MC205395 ... Lentivirus constructs (pLKO.1) harboring short hairpin RNA (shRNA) to specific ZBTB32 (cat
Showing 1-1 of 1 papers.
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