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Usp2 (NM_016808) Mouse Untagged Clone

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SKU
MC203948
Usp2 (untagged) - Mouse ubiquitin specific peptidase 2 (Usp2), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $577.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Usp2
Synonyms B930035K21Rik; Ubp41
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC017517
CTCCAGCCTCTGGAGGCTACACAGCAGTCTTTTCCCTTCGGGGAAAGAAGCCCCCAGGCTCCATCCAGTG CCCCCAACACAGCCCATGAGGCTCCCAGTACCCACTGAGTACCACCCTGAAGGATGTCCCAGCTCTCCTC CACCCTGAAGCGCTATACAGAATCGTCCCGCTACACAGATGCCCCCTATGCCAAACCGGGCTATGGCACC TACACCCCTTCTTCCTATGGGGCCAACCTGGCCGCCTCCTTCCTGGAGAAGGAAAAACTTGGTTTCAAGC CGGTCTCCCCCACCAGCTTCCTCCCACGGCCCCGCACCTACGGCCCCTCCTCCATTCTGGACTGTGACAG GGGCCGCCCCCTGCTGAGATCTGACATCATTGGGAGTAGTAAGCGGTCAGAGAGCCAGACCCGTGGCAAT GAAAGGCCCTCGGGCAGTGGACTCAACGGAGGCAGTGGATTTTCTTATGGAGTTTCCAGCAACTCCCTCA GCTACCTGCCCATGAATGCCAGAGACCAGGGGGTGACCCTGAGCCAGAAGAAATCGAACAGCCAATCAGA CCTGGCCCGGGATTTCTCCAGCCTCCGGACTTCAGATGGCTACCGGACTTCAGATGGCTACCGGACCTCA GAGGGCTTTAGGATAGACCCTGGAAACCTGGGTCGCAGCCCCATGCTGGCCCGCACGCGTAAGGAGCTCT GTGCCCTGCAGGGCCTCTACCAAGCAGCCAGCCGTTCTGAGTACCTAACAGACTATCTGGAGAACTATGG TCGAAAGGGCAGTGCACCACAGGTGCTCACGCAAGCCCCTCCTCCCTCCCGAGTCCCTGAAGTCCTCAGT CCCACCTACCGACCAAGTGGCCGTTACACACTGTGGGAGAAGAGCAAGGGCCAGGCCTCTGGGCCCAGCC GCTCCAGTTCTCCAGGGCGAGACACCATGAATTCAAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCT TGGGAACACGTGCTTCATGAACTCAATTCTTCAGTGCCTGAGCAACACCCGAGAGCTGAGAGATTACTGC CTCCAGAGGCTGTACATGCGGGACCTCGGCCACACCAGCAGCGCTCACACGGCCCTCATGGAAGAGTTTG CAAAACTAATCCAGACCATATGGACGTCGTCCCCCAATGATGTGGTGAGCCCATCTGAGTTCAAGACCCA GATCCAGAGATATGCGCCACGCTTCATGGGCTATAATCAGCAGGATGCTCAGGAATTCCTTCGTTTCCTT CTGGATGGTCTCCACAATGAGGTGAACCGGGTGGCAGCAAGGCCTAAGGCCAGCCCTGAGACCCTTGATC ATCTCCCTGATGAAGAAAAGGGGCGACAGATGTGGAGGAAGTATCTGGAAAGGGAAGACAGTCGGATTGG GGATCTCTTCGTTGGGCAGCTGAAGAGCTCCCTCACATGCACCGATTGTGGCTACTGCTCTACAGTCTTC GATCCCTTCTGGGATCTCTCGTTGCCCATCGCAAAGAGAGGTTACCCTGAGGTGACGTTAATGGATTGTA TGAGGCTCTTCACCAAAGAGGACATATTGGATGGTGATGAGAAGCCAACTTGCTGCCGCTGCCGAGCCAG AAAACGATGCATAAAAAAGTTCTCTGTCCAGAGGTTCCCAAAGATCTTGGTGCTCCACCTGAAGCGATTC TCAGAATCCAGGATACGAACCAGCAAGCTCACAACATTTGTGAATTTCCCACTAAGAGACCTGGACTTGA GAGAATTTGCTTCAGAAAACACCAACCATGCTGTTTACAACCTGTATGCTGTGTCCAATCACTCCGGAAC CACCATGGGAGGCCACTATACAGCCTACTGCCGAAGTCCGGTTACAGGCGAATGGCACACTTTCAATGAT TCCAGTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTATTTGCTCTTCTATGAACTGG CCAGTCCACCCTCCCGTATGTAGCATTGAGGAGCTGCGGCCCTTCCCTCTTCCCTGTGGTGGCCCCACGT CCTAAGTTTTTTTTAAAAAATTCAGAAAAAAAAAAAAAAAACACCACAAAAAAAAAAAACCAAAAAAAAA AAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_016808
Insert Size 1860 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC017517, AAH17517
RefSeq Size 2115 bp
RefSeq ORF 1860 bp
Locus ID 53376
UniProt ID O88623
Cytogenetics 9 A5.1
Summary Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity). Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity). Has no deubiquitinase activity against p53/TP53 (By similarity). Prevents MDM2-mediated degradation of MDM4 (By similarity). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (PubMed:23213472, PubMed:25238854, PubMed:26756164). Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (PubMed:23213472). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (PubMed:25238854).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. Variants 1 and 3 encode the same protein (isoform Usp2-69).
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Documents
Resources
"NM_016808" in other vectors (3)
SKU Description Size Price
MR209481 Usp2 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 2 (Usp2), transcript variant 1 10 ug
$289.00 $576.00
MR209481L3 Lenti ORF clone of Usp2 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 2 (Usp2), transcript variant 1 10 ug
$876.00
MR209481L4 Lenti ORF clone of Usp2 (mGFP-tagged) - Mouse ubiquitin specific peptidase 2 (Usp2), transcript variant 1 10 ug
$876.00

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