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Inpp5e (NM_033134) Mouse Untagged Clone

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SKU
MC203470
Inpp5e (untagged) - Mouse inositol polyphosphate-5-phosphatase E (Inpp5e), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $603.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Inpp5e
Synonyms 72kDa; 1200002L24Rik; mKIAA0123
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC052717
AGCCAGGGTGACCACTAGACTGAGCTGGGGCGAACCCTGGGCGGGGGCGGGGGCCGAGGCACGGGGCCGC AGTCGTTGTTCCAGCTGCCACCGTGACTGGCCTAGGTCCGCCTCCGCCAGCGCTGGGCCCGGCTGAATCA GACCTCGGGCGTCCGGCGGCGGGTCTGCTGCCGAACGGAGGGCGCAGGTGAGCCTAGGACGCTGAGCGCG CTGCCCTACTCTGCCTCGGCGGGGTGGAGGGCTGGCCGGGGGTGCTGGACAGACTTGTGCAGCTCCGTGA AGTGCAGCCCCGACCCCCTGGCGCCTGTTCTCACGAAGTCGCTTTTAAGACTAGCGAGCTGACTTCGGGG AGCCAAGAGATTTTTCTGCGTTTCCTCAGGCATCGGTGAGAACTCTCAAGTCTCCAGCCATGCAGCTACA GGCCCTCCTCCAAAAGGACCGCCCCATGCCACAAAGGGAGCCACTCGAGTTTTCATGGCATCGAGCTGGC GAACTCTGACGTGCATCCTTGACAACAAGAGTTGCTTGTTAACCTCCCAGGGAGGATCCCTTTCCTTAGG AGGGCCAGGCTGAGGGGGTCAGGGCTGGCCAGGTCTGGACTATGCCATCCAAGTCAGCTTGCCTGCGTCA CACTGAGGCACCTGGGCAGCTAGAAGGCAGAATGCTCCAGGGACAGCCTCCGAACACGGAAAAGAAGCTC ATCCCAACACCAGGCTTCTTACCAGCCTCTGACTCTCAGGGTTCAGAGACAAACCCCATGCCTCCTTTCA GCATTCCAGCAAAAACAAGCAACCAGAACCCACAAACTAAGGCAAATCTCATCACCCCACAGCCACCCAT TAGACCCAAGTTGGAGCGAACCCTGTCCCTCGATGACAAGGGCTGGAGAAGGAGGCGTTTCCGGGGTAGT CAGGAGGATCTGACTGTCCAGAATGGGGCCAGTCCCTGCAGGGGCTCCTTGCAAGACTCAGTAGCCCAGT CCCCTGCCTACAGCCGTCCCCTGCCCTGCCTCAGCACATCCTTGCAGGAGATACCTAAGTCCCGAAGGGC CACAGGCAGTGAGGGAGGGAGCCCATCCTTGTGGAGTGACTGTCTTTCTGGAATGATCAGCACCTCCTTG GACCTCCTGCACAGAGATGCTGCCTCAGGTGGGCCCCCCTCCAGGTTGGCAAGTTTGCATGCCTCACACA CACCACCAGCTATGGACCTCAGCATAGCCTCCAGCTCCCTGAGAACAGCAAACAAGGTTGACCCCGAGCA CACGGACTACAAGCTCCGCATGCAGACCAGACTGGTCAGGGCCCACAGCAACCTGGGCCCTAGCAGACCC CGGAGCCCCTTGGCTGGTGACGATCACTCCATTCACTCAGCCAGATCTTTCAGCCTTCTGGCCCCCATCC GCACCAAGGACATCAGAAGCAGGAGCTATCTGGAGGGAAGTCTTCTGGCCAGTGGGGCCCTGCTAGGAGC AGAAGAGCTGGCCAGGTACTTCCCAGACCGAAACATGGCTCTCTTCGTGGCTACCTGGAACATGCAGGGC CAGAAGGAGCTCCCAGCGAGCCTGGATGAGTTTCTGCTCCCCACCGAGGCTGACTACACTCAGGACCTGT ATGTCATTGGAATTCAGGAGGGCTGCTCTGACAGGCGGGAGTGGGAGACACGCCTGCAGGAGACACTGGG CCCTCAGTATGTACTGCTGTCATCAGCAGCACATGGGGTCCTGTACATGTCCCTGTTTATCCGTAGGGAC CTCATCTGGTTCTGCTCAGAGGTCGAGTACTCCACAGTAACTACACGCATCGTGTCTCAGATCAAGACCA AGGGGGCCCTGGGCGTCAGCTTCACCTTTTTTGGCACCTCCTTCCTCTTCATCACATCTCACTTCACCTC TGGAGATGGGAAGGTAGCAGAGCGGCTACTGGACTACAGCAGAACCATCCAAGCCCTAGCCCTGCCCCGG AATGTGCCAGACACAAATCCCTACCGCTCTAGTGCAGGGGATGTCACTACCCGGTTTGATGAGGTCTTCT GGTTTGGGGACTTCAACTTCCGCCTGAGTGGTGGACGAGTGGCTGTGGAGGCCTTCCTGAAGCAGAAACC TGAGGTGGATGTGCTGGCTCTCCTCCAACACGACCAGCTCACCCGGGAGATGAAGAAAGGGTCCATCTTC AGGGGCTTTGAGGAGGCAGAGATTCACTTTCTTCCATCCTACAAGTTTGACATTGGGAAGGACACCTACG ACAGCACCTCCAAGCAAAGGACACCCTCCTACACAGACCGAGTCCTATACAAAAGCCGTCACAAGGGTGA CATCTGTCCCATGAAGTATTCCTCTTGTCCTGGGATCAAGACTTCAGACCACCGTCCTGTGTATGGCTTG TTCCAGGTGAAAGTGAGGCCAGGACGAGACAACATCCCATTAGCTGCCGGCAAGTTTGACCGAGAATTGT ACTTGATAGGAATTAAAAGGCGGATTTCAAAGGAGATTCAGAGACAGGAAGCACTGAAGAGCCAGAGCTC CAGTGCAGTTTGCACCGTGTCCTGAAGATTCCAGACCAGGACTGGCCACAGCAACGGGGGCCAAGGTCAC TGCAAAGGATGGGCTTCTTCTCAGCTGCTCCTGTAATGCTGGGATTCTAATCCACCACGGCCAGGGGCTC CCTGCTCTCACCACAGACCCAGCCCTCAGGACATTTATACCATGTTCCCACAGGCACATGGCAGGGCATG TGGACACCAAGCAGCAGTTTTGTCCTCAGGAGTAGTCCAGACTTCTTGCCCTTGAGCACTGCTGTCTCTT CAAACTCCTTTTATTTATTCCTGTTCTCATGCTAGAGCCCAGGTTCTCCATCTGTATCCTCCTAGTAGCT CCTTCCCTCCAGGGAAGGATCAGGCAACCTCTGGTATCCTAGATGCTCAATGCCTAGAACCTGCCAGGAC TTCAATTCTGGGATATTCCCTACAGACATTTTGACAGGCTTTGAGGTTCTACATCCTGGTCCCAGGCTCC CAACCCCCAGTTGAGTAGGGACCTCTCCACTCTACAGGCCAAGCCTAGCTATATTAAACTCATAGTTTTT AGGAGCTTCAGACCATTTGCTTTTGTGCTGCTAGTCCCGGAAAGGCATGAGAAATTGTTACCAGACAGCA CCTTGCTGGGTTTCACTCCTTGAAGAAAGCTTAGGAGCATCATTGCTCTCAATCTTACCCCACCCCTTCA GACATAGCCTTGAATGTACAGACACCTAGGGGCATCCACTCTAGTCTTCTAAATCATTTATTTTTCCATA TTTATATTTTTAAATAAATAAGGATATATTCAATTGTAAACTCTTTCTGGCCAAAGAGTGAACCTTGGAA GAAGTCTGCATGCATCTTGGGGCCTCAGGACACCCCAGGGTTTTAAAAGGACATAGAAGTCAAAGGCCAA ACATTTGGTCTCAGCTGTCACCTGCTACTCAGTCCCATCCCAGGTCTTTCTGCTAGGTACTGTCCCTGCT CACAGCCAGAAGCAGCAATAGCTGGAAAAAGTGACGACTTGGGACTCCAGTGAACCATAATAAACCAACG AATAAGGTGAAACTCAACAGCATCAGCTTCTCCAATATGGAAAGTCACCTGCTTCAATAAACCACTGTGA ACTGCAAATTCTACTGGGAAGACAGCCTTTTCTCTCCCAGACAGGCTGCCCTGGACTTCATAATCCTGCC TCAACTATTCCTGGCTTGAATAAGGTTTGATGTGGTAAGAGTTCCAGACAGGTTTCTCTGTAGCCCCGGC TGTCCTGGAACTCTCTGTAGACCAGGCTGGCCTCGAACTTGGAGAACCACCTGCTTCTGCCTCCTGAATG CTGGGATTAAAGGCGTGCACTACCACAGCCCAGCAGTTTGGTGCTTCTTTGTGGACATTCTCTGGCACCT TAGAAAAGACTGAGGTAATCCTGGAACTTCCCAGTTTGGCCCAAGATCAAGATCATAAAGTCTTAGGGGT CGGGGGGCAAAGTACAGCCATAGCAATTTACATTTAGATAGCCTTTACATTTAGAGGGCATTTCAGTTGG AAAAGTTAAACACGTAAACTAGTGCAGATGAAGTGGGAAATTAAAACTGGACTTGTAAATAATTGTGAGA TACAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_033134
Insert Size 1944 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052717, AAH52717
RefSeq Size 4155 bp
RefSeq ORF 1944 bp
Locus ID 64436
UniProt ID Q9JII1
Cytogenetics 2 A3
Summary Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Specific for lipid substrates, inactive towards water soluble inositol phosphates (By similarity) (PubMed:10806194). Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability (PubMed:19668215).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (a).
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Resources
"NM_033134" in other vectors (4)
SKU Description Size Price
MG209731 Inpp5e (tGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase E (Inpp5e) 10 ug
$803.00
MR209731 Inpp5e (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase E (Inpp5e) 10 ug
$289.00 $603.00
MR209731L3 Lenti ORF clone of Inpp5e (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase E (Inpp5e) 10 ug
$903.00
MR209731L4 Lenti ORF clone of Inpp5e (mGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase E (Inpp5e) 10 ug
$903.00

file-alt Citations

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