Mapkapk2 (NM_008551) Mouse Untagged Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Mapkapk2 |
| Synonyms | AA960234; MAPKAP-K2; MK-2; MK2; Rps6kc1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | AscI-NotI | |
| ACCN | NM_008551 |
| Insert Size | 1161 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC063064, AAH63064 |
| RefSeq Size | 2586 bp |
| RefSeq ORF | 1161 bp |
| Locus ID | 17164 |
| UniProt ID | P49138 |
| Cytogenetics | 1 E4 |
| Summary | Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (By similarity). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress following ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (By similarity). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
"NM_008551" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR206027 | Mapkapk2 (Myc-DDK-tagged) - Mouse MAP kinase-activated protein kinase 2 (Mapkapk2) | 10 ug |
$289.00
$457.00
|
|
| MR206027L3 | Lenti ORF clone of Mapkapk2 (Myc-DDK-tagged) - Mouse MAP kinase-activated protein kinase 2 (Mapkapk2) | 10 ug |
$757.00
|
|
| MR206027L4 | Lenti ORF clone of Mapkapk2 (mGFP-tagged) - Mouse MAP kinase-activated protein kinase 2 (Mapkapk2) | 10 ug |
$757.00
|
|
Citations
Degradation of MK2 with natural compound andrographolide: A new modality for anti-inflammatory therapy
Tran, Gan, Liao et al
Pharmacol Res (2023) 194, 106861 DOI: 10.1016/j.phrs.2023.106861
Tran, Gan, Liao et al
Pharmacol Res (2023) 194, 106861 DOI: 10.1016/j.phrs.2023.106861
Cited by 12
AI Summary: OriGene’s ORF cDNA clone (CAT# MC203417) was used in this study to investigate the role of MK2 in inflammation and the mechanism by which andrographolide induces MK2 degradation.
Snippet: The p38 MAPK -MK2 signaling axis functions as an initiator of inflammation. Targeting the p38 MAPK -MK2 signaling axis represents a direct therapeutic intervention of Save;siRNAs for MK2 (m) (CAT# sc-35856), TTP (m) (CAT# sc-36761), and scramble siRNA (CAT# sc-374305) were purchased from Santa Cruz Biotechnology (Dallas, TX). ORF cDNA clone (CAT# MC203417) came from Origene (Rockville, MD)
Showing 1-1 of 1 papers.
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