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Nek7 (NM_021605) Mouse Untagged Clone

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SKU
MC203059
Nek7 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nek7
Synonyms 2810460C19Rik; AU020186
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC037697
CCACGCGTCCGCGCGGGCCGAGCAGGGTGGGAGCCGGCGCCTGCAGTGGCGCGGGGAGAGGCGATCGGCC CCGGATCCCGCAGCCCTGCAGCCCGCGTAGACCGAGCTGCCCCGGCGCCCCGAATCCTGAAGTCCCAGTT GAGTCAGAATGGATGAACAATCACAAGGAATGCAAGGGCCGCCCGTTACTCAGTTCCAGCCACAGAAGGC ATTACGGCCAGATATGGGCTATAATACTTTAGCCAACTTCCGAATAGAAAAGAAAATTGGTCGTGGACAA TTTAGTGAAGTTTATAGAGCATCCTGTCTCTTGGATGGAGTGCCGGTAGCGTTAAAAAAAGTACAGATAT TTGATTTAATGGATGCCAAAGCACGTGCTGATTGTATCAAAGAAATAGACCTCCTTAAGCAACTCAACCA TCCAAATGTAATTAAATACTATGCATCATTCATTGAGGATAATGAGCTGAACATAGTTTTGGAGTTAGCA GATGCTGGTGACCTCTCCAGAATGATAAAGCACTTTAAGAAACAAAAGAGGCTAATCCCTGAGAGAACCG TTTGGAAATACTTCGTTCAGCTCTGCAGTGCACTGGACCACATGCATTCTCGAAGAGTCATGCACAGAGA TATAAAACCAGCTAATGTGTTCATTACAGCCACTGGGGTAGTAAAACTCGGAGACCTTGGGCTTGGTCGG TTTTTCAGCTCCAAAACCACAGCTGCACATTCTTTAGTGGGTACACCTTACTACATGTCTCCAGAGAGAA TACATGAAAATGGATACAACTTCAAGTCTGACATCTGGTCTCTTGGCTGTCTGCTATATGAGATGGCTGC ACTGCAGAGTCCTTTCTACGGCGACAAGATGAACTTGTATTCTCTGTGTAAGAAGATAGAGCAGTGTGAC TACCCGCCTCTCCCGTCAGATCACTATTCGGAGGAGCTACGACAGCTAGTTAATATATGCATCAACCCAG ATCCAGAGAAGCGACCCGACATCGCCTATGTTTATGATGTGGCAAAGAGGATGCATGCATGTACCGCAAG CACCTAAACTGTACAAGATCCTGAAGACGGCAACCAAGATAACTTAAAAGTGTTTTTGTGCAGATCATAC CTCCCCGCTTATGTCTGGGTGTTAAGATTACTGTCTCAGAGCTAATGCGCTTTGAATCCTTAACCAGTTT TCATATGAGCTTCATTTTTCTACCAGGCTCAATCACCTTCCCAATCCACAACTTTGGGATGCTCAGATGG CACCAAGAATGCAAGCCCAACAAGAGTTTTTCGTTTGAGAATTGTTTCGAGTTTCTGCTGATAGACTGTG TTTATAGATAGTCAGTGCCCGATGGTGAAGCACACACACATAGGCACATGTCCAGAGCGATGCAGAACCT GAGGAAGGACCTGGGCATTTGACTTGTTTGCTTTTAAGTCACTTAATGGACGTTGTAGTGGACATGATTG TGAACTTCTGATTTTTTTCTTTTAAGTTTCAAGTACATGTTTTAGTTCTTAGCATTAGAGATCTCAAATA TCATTCTTATAAGACATGCAGACATAAACTTTTTGAGAAAGATTTAAAATTTTTAGTTTATACATTCAAA ATGCAACTATTAAATGTGAAAGCATAGAGGTCAAAATGTGAGTTGGACACTGAAGTCTATGTTTTAATGC CTTTGAAAGCCTTTTTTTTGTGTGTGTTTAAATGGTATAAATGAACCCATTTTAAAACGTGGTTAAGGAC TTGTTTGCCTGGCGTGATAGTCATGTTTAACATGCACAAGGCTTTGTGTTTTTATTGTACATTTGAAGAA TATTCTTGGAATAATCTTGCAGTAGTTATAGTTCAATTTCTTTACAAATCTAAATACACTTAACTCATAA CTATACACTGTAATGCAAGCATATATTGTTATTCATATATTGAAGTTTTGATCAGTTCCTCTTCAGAATC TTTTTTATCCAAGTTACTTTCTTATTTATATTGTGTGTGCATTTCATCCATTAAATGTTTCAGATTTTCT GAGAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_021605
Insert Size 909 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC037697, AAH37697
RefSeq Size 2059 bp
RefSeq ORF 909 bp
Locus ID 59125
UniProt ID Q9ES74
Cytogenetics 1 60.82 cM
Summary Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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"NM_021605" in other vectors (4)
SKU Description Size Price
MG204231 Nek7 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7) 10 ug
$500.00
MR204231 Nek7 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7) 10 ug
$289.00 $300.00
MR204231L3 Lenti ORF clone of Nek7 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7) 10 ug
$600.00
MR204231L4 Lenti ORF clone of Nek7 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7) 10 ug
$600.00

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