Gars (NM_180678) Mouse Untagged Clone

SKU MC202967

Gars (untagged) - Mouse glycyl-tRNA synthetase (Gars), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gars
• Synonyms: Gena201; GENA202; Nmf249; Sgrp23
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021747 sequence for NM_180678; GCACCGCCTAGGCGGCGTGCTCATGCCCTGTCTGCTCCCCTCGCTGCTCAGAGCCACCCGCGCCGCGCTG CCGCTCCTGTCGCCGCCCCGAGTGGTCGCAGCGTCCGCGTCTCAGCGGCTCCTCAGCGCTCCCGCGCAGC CCGCCGCCTCCCGGAGCAGCATGGACAGCGCGGAGGAGCTGCTGGCCCCACTGCGGCTAGCCGTGCGCCA GCAGGGAGACTTTGTACGGAAGTTAAAAGAAGATAAAGCCCCACAAGTGGATGTGGACAGAGCAGTAGCT GAGCTCAAGGCCCGGAAGAGGGTTCTGGAAGCAAAGGAGCTGGCATTGCAGCCCAAAGATGACATCGTAG ATAGAGCAAAAATGGAAGATACGTTGAAGAGGAGGTTTTTCTACGATCAGGCTTTTGCTATTTATGGAGG TGTCAGTGGATTGTATGACTTCGGGCCGGTAGGATGTGCTTTGAAGAACAATATCATCCAGGCCTGGAGG CAGCACTTTATCCAAGAGGAGCAGATCCTGGAGATTGACTGCACCATGCTCACCCCTGAGCCAGTTTTAA AGACCTCTGGCCACGTAGACAAATTTGCTGACTTCATGGTGAAGGACGTGAAGAACGGAGAGTGCTTCCG AGCAGACCACCTGTTGAAAGCTCATTTACAGAAACTGATGTCAGATAAGAAGTGCTCTGCTGAGAAGAAG TCAGAGATGGAAAGTGTCTTGGCCCAGCTTGATAACTATGGACAACAAGAACTTGCGGATCTTTTTGTGA ACTATAATGTAAAATCTCCCACCACTGGCAATGACCTGTCCCCTCCGGTACCTTTTAACTTAATGTTCCA GACCTTCATTGGGCCTGGAGGAAATATGCCTGGATATCTGAGACCAGAAACTGCACAGGGAATTTTCCTG AATTTCAAACGACTTTTGGAATTCAACCAAGGGAAATTGCCTTTTGCTGCTGCCCAGATTGGAAACTCCT TCAGAAATGAGATCTCACCTCGGTCTGGACTGATCCGAGTCAGGGAGTTTACAATGGCAGAGATTGAGCA CTTTGTAGATCCCACTGAGAAAGACCATCCCAAGTTCCAAAGTGTGGCCGACCTCTGCCTTTATTTGTAC TCAGCAAAAGCCCAGGTCACTGGACAGTCTGCTCGGAAGATGCGTCTGGGAGATGCTGTTGAACAGGGTG TGATTAACAACTCAGTATTAGGCTATTTTATTGGCCGCATCTACCTCTACCTCACGAAGGTTGGAATATC TCCTGATAAACTCCGCTTCCGGCAGCATATGGAGAATGAGATGGCCCATTATGCCTGCGACTGCTGGGAT GCCGAGTCCAAAACGTCCTATGGCTGGATTGAGATTGTTGGATGTGCTGACCGTTCCTGCTACGACCTCT CCTGTCATGCTCGAGCCACCAAAGTTCCACTAGTAGCTGAGAAACCTCTGAAAGAACCCAAAACAGTTAA CGTTGTACAGTTTGAGCCCAACAAGGGCGCCGTGGGCAAGGCGTACAAGAAGGATGCAAAGCTAGTGCTG GAGTATCTCAGCGCCTGTGATGAGTGCTACATTTCAGAGATGGAGCTGCTGCTGAGTGAGAAAGGGGAAT TCACTATTGAAACTGAAGGAAAAACATTTCAGTTAACGAAAGACATGGTCAGTGTGAAGAGATTCCAGAA AACACTGCATGTGGAAGAAGTTGTTCCGAGTGTAATTGAGCCCTCCTTTGGCTTGGGCAGGATCATGTAT ACCATATTGGAACATACATTCCATGTCCGAGAGGGAGATGAACAGAGAACGTTCTTCAGTTTCCCTGCTG TGGTTGCTCCATTCAAATGTTCTGTCCTTCCACTGAGCCAGAACCAAGAGTTTATGCCATTTGTCAAGGA ATTATCCGAAGCTCTGACCAGGAACGGCGTGTCTCATAAAGTCGATGACTCCTCTGGGTCTATTGGAAGG CGCTATGCAAGAACTGATGAGATTGGCGTGGCTTTCGGCATCACTATTGACTTTGATACAGTGAACAAGA CGCCCCACACTGCAACTCTGAGGGACCGAGACTCCATGAGACAGATAAGGGCAGAGGTCTCTGAGCTGCC CAGTGTGGTCCGCGATCTGGCCAACGGCAACATTACCTGGGCTGATGTGGAGGCCAGGTACCCACTCTTT GAAGGGCAAGAGACTGGCAAGAAGGAGACAGTAGAGGAATGAGAACAGCTGGCAGCTTATGACCATCGTG CTAATAAATAACTCATCATGTCCACTACACAGAGCATTGTGACTGCATCCAGGGACGGTTTTTCCTCAGT GGCTGCTTGGTTTTACTCCCACAATTAAAGTTGAAGGAATTCTGAACACATTTATAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_180678
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC021747, AAH21747
• RefSeq Size: 2380 bp
• RefSeq ORF: 2190 bp
• Locus ID: 353172
• UniProt ID: Q9CZD3
• Cytogenetics: 6 27.29 cM
• Summary: Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.[UniProtKB/Swiss-Prot Function]