Hmgcl (NM_008254) Mouse Untagged Clone

SKU MC202939

Hmgcl (untagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Hmgcl
• Synonyms: AW476067; HL
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025440 sequence for NM_008254; CCACGCGTCCGGAAGATGGCGTCAGTGAGGAAGGCTTTCCCGCGGAGGCTGGTGGGCTTGACGTCCCTCC GGGCTGTGAGCACCTCCTCCATGGGCACTTTGCCAAAGCAGGTGAAGATCGTGGAAGTCGGTCCCCGAGA TGGGCTGCAGAATGAAAAGAGTATTGTACCCACCCCAGTGAAGATCAGGCTGATCGACATGCTTTCCGAA GCAGGGCTCCCCGTGATCGAGGCCACCAGCTTTGTTTCTCCCAAGTGGGTGCCACAGATGGCTGACCACT CTGACGTCTTGAAGGGCATTCAGAAGTTTCCCGGCATCAACTACCCAGTCCTGACTCCAAACATGAAAGG CTTTGAGGAAGCGGTAGCTGCGGGTGCCAAGGAAGTGAGCGTCTTCGGTGCTGTGTCTGAGCTCTTCACC CGGAAGAACGCGAACTGCTCTATAGAGGAGAGTTTCCAGCGCTTTGCTGGAGTCATGCAGGCGGCCCAGG CCGCCAGCATCTCTGTGAGAGGGTATGTCTCCTGTGCCCTCGGATGCCCCTACGAGGGGAAGGTCTCCCC GGCTAAAGTTGCTGAGGTTGCCAAGAAGTTGTACTCGATGGGCTGCTATGAGATCTCCCTTGGGGACACC ATCGGCGTAGGTACCCCAGGACTCATGAAAGACATGCTGACTGCTGTCATGCATGAAGTGCCTGTGACAG CATTGGCTGTCCACTGCCATGATACCTATGGTCAAGCTCTGGCCAACACCTTGGTGGCCCTGCAGATGGG TGTGAGTGTTGTGGACTCCTCTGTGGCAGGACTTGGAGGCTGTCCCTATGCAAAAGGGGCATCAGGGAAC TTGGCTACTGAGGACCTGGTCTACATGCTGAATGGCTTAGGGATTCACACGGGTGTGAACCTCCAGAAAC TTCTAGAAGCTGGGGACTTCATCTGTCAAGCCCTTAACAGAAAAACTAGTTCCAAAGTGGCACAGGCCAC CTGCAAGCTCTGAGACCCTCGTTCGCCTGAACCAGAACGGAGGGAATTGGGTGTATACAATGATTCCTGG ATGAAGAGATGGAATGAGAGCAAATGAGCCGGCATCACAGAGGTCCCTGTCCCTGATGATAAGGGCTAGA GCTGCCTGGCCTGGGCCAGCTCCCCAGAGCTGTGCCTAAGCACTTGCTTGGGATGGCCCTGGGTGAGTCT GCCTGCCAACAGAGCTGATGTCCACCGCCGCAGGGCCTTTGTTCTACCTCTGAGGACAGAGAGCAGTTTC CCCTTATGCCAGCAGAGCATTTGCTGGAATGGTGGGGGTTGATCTGCATCTGTGGTCATCTGCCAACGAA ATCTCCACTCTGTGTGTGATTTTTTTGAAAACAGCTTATGTAATTAAAGGTTTAATTTTCTAATATAAAA AAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008254
• Insert Size: 978 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC025440, AAH25440
• RefSeq Size: 1412 bp
• RefSeq ORF: 978 bp
• Locus ID: 15356
• UniProt ID: P38060
• Cytogenetics: 4 68.14 cM
• Summary: Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.[UniProtKB/Swiss-Prot Function]