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Eif5a (NM_181582) Mouse Untagged Clone

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SKU
MC202889
Eif5a (untagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 5, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Eif5a
Synonyms AA410058; D19Wsu54; D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1; eIF-5A1; Eif4d; Eif5a1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC024899 sequence for NM_181582
GCGGCGGCGGCGGCGGCGGCAGCACAGTAGCGGGTTCGGAGGCAGCAGTTGGGCTCGCGGCGAGCGGACC AGGTCAAGTCAGTGCGTTCGCGCGAGTTGGAATCGAAGCCTCTTAAAATGGCAGATGATTTGGACTTCGA GACAGGAGATGCAGGGGCCTCAGCCACCTTCCCAATGCAGTGCTCAGCATTACGTAAGAATGGTTTTGTG GTGCTCAAAGGCCGGCCATGTAAGATCGTCGAGATGTCTACTTCGAAGACTGGCAAGCATGGCCATGCCA AGGTCCATCTGGTTGGCATTGACATTTTTACTGGGAAGAAATATGAAGATATCTGCCCGTCGACTCATAA TATGGATGTCCCCAACATCAAACGGAATGACTTCCAGCTGATTGGCATCCAGGATGGGTACCTATCCCTG CTCCAGGACAGTGGGGAGGTACGAGAGGACCTTCGTCTGCCTGAAGGAGACCTTGGCAAGGAGATTGAGC AGAAGTATGACTGTGGAGAAGAGATCCTGATCACAGTGCTGTCTGCCATGACAGAGGAGGCAGCTGTTGC AATCAAGGCCATGGCAAAATAACCGGCTTCCAGGGTGGCGGTGGTGGCAGCAGTGATCCATGAGCCTACA GAGGCCCCTCCCCCAGCCAGGTTCTGCTCTGGCTGGGCCCTTGGCTGGACTCCTATTCAAATTTATTTGA CGTTTTATTTTGGTTTTCCTCACCCCCTCAAACTGTCGGGGAGACCCTGCCCTTCACCTAGCTCCCTTGG CCAGGAATGAGGGAGCCATGGCCTTGGTGAAACTCCCTGCCTCTTCTCTCGCAGCCCTGATGGGGGAAAG GGAGTTGGGTACTGCTTGTGGTTTAGGTTCCCCTCTCCTTTTTTTCTTTTTAATTCAATTTGGAATCAGA AAGCTGGATTCTGGTAAATGGTCTTGTGCCCTTTATCCTACTCATCCCCGATCTGGTCCCCTGTTCTCCA TAGTCCTTCACCCCCAAGCACCACTGTACAGACTGGGGACCAGCCCCTTCCCTGCCTGTGTCTCTTCCCA AACCCCTGTAGGGGTGGTGAGAAGAGGAGGGGGGGAGGGGACACGATCCCTCCTCAGGCATCTGGGAAGG CCTTGCCCCCACGGGCTTTACCCTTTCCTGTGGGCTCTCTCCCTGACACATTTGTTAAAAATCAAACCTG AATAAAACTACAAGTTTAATATGAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_181582
Insert Size 465 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC024899, AAH24899
RefSeq Size 1232 bp
RefSeq ORF 465 bp
Locus ID 276770
UniProt ID P63242
Cytogenetics 11 B3
Summary This gene encodes an elongation initiation factor, which participates in protein synthesis. The encoded protein also plays roles in mRNA metabolism, cell proliferation, and cell cycle control. This protein contains a modified lysine residue called hypusine, which appears to be necessary for its function. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 2, 5, and 19. provided by RefSeq, Oct 2009
Transcript Variant: This variant (5, also referred to as variant B) differs in the 5' UTR compared to variant 1. Variants 1-9 encode the same protein.
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"NM_181582" in other vectors (4)
SKU Description Size Price
MG201137 Eif5a (tGFP-tagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a) 10 ug
$350.00
MR201137 Eif5a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 5 10 ug
$289.00
MR201137L3 Lenti ORF clone of Eif5a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 5 10 ug
$450.00
MR201137L4 Lenti ORF clone of Eif5a (mGFP-tagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 5 10 ug
$450.00

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