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Trmt5 (NM_029580) Mouse Untagged Clone

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SKU
MC202840
Trmt5 (untagged) - Mouse TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (Trmt5), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $467.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trmt5
Synonyms 2610027O18Rik; mKIAA1393
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC012521 sequence for NM_029580
CGGACGCGTGGGCTGCACCAGAGCATGAGAATCGTATGGAAGCTATTTGGATTCTCAAGAAGACTTTTGC AAGTGGAATGGTGTCACCCCAGTGAATCAATATTGTTGTTTACCCTGGTGCCGAGACTTAGGAAAGCGCC TTCTGTTTTCTTGTTGGGTCAAAGACAAGGACTCTCAACCATGCCAGAAATAGAAGCAAGTGTGAGAGAT AGTGAATTATTTTCACCACCCTCTGACGTTCGAGGAATGAGAGAACTTGATAGAACAGCTTTTAAGAAGA CAGTGTCCATCCCTGTGCTTAAAGCGAGGAAAGAAGTAGTCAACAGATTGATGCGAGCCCTCAGGAGAGT AGCCCTGCAGCGCCCGGGCATAAAGCGAGTGATTGAGGATCCCAAAGATGAAGACAGTAGGCTGATCATG TTGGACCCCTACAGGATGCTTACTGCTGATTCCTTTGACAAAGCAGAACTCGGAGTTTTAAAAGAACTTG ATGTCAGCCCACAGTTATCCCAGTACAACTTGGAACTAACATATGAAAATTTTAAGTCAGAAGAAATTTT GAAAGCTGTGCTTCCTGAAGGTCAAGATGTAACCTCTGGATTCAGCAGGGTTGGGCATATTGCACACCTG AATCTCCGAGATCACCAGCTGCCCTTCAAGCATTTAATTGGCCAAGTTATGGTTGACAAAAACCCAGGAA TCACCTCAGCAGTAAATAAAACCAGCAACATTGACAATACGTACCGAAATTTCCAAATGGAAGTGCTGTG TGGAGAGGAAAATATGCTGACCAAGGTTCGAGAAAACAACTACACGTATGAGTTTGATTTCTCTAAAGTC TACTGGAATCCTCGTCTCTCTACTGAACATGGCCGGATCACTGAACTTCTCAACCCTGGAGATGTCTTGT TTGATGTTTTTGCTGGGGTTGGGCCCTTTGCCATTCCAGCAGCAAGGAAAAACTGCACAGTGTTTGCCAA TGATCTCAATCCTGAGTCCCATAAATGGCTGTTGCACAATTGTAAACTGAATAAAGTTGACCAGAAAGTG AAAGTCTTTAACATGGATGGAAAAGACTTCATCCAAGGGCCAGTCAGAGAAGAGTTAATGCTTCGGCTAG GATTGTCAGCTGAAGCTAAACCCTCTGTCCACATTGTCATGAACTTGCCAGCTAAGGCCATAGAGTTTCT CAGTGTTTTCAGATCACTTTTGGATGGGCAGCCTTGCAGCACGGAGCTCCTCCCCACAGTGCACTGTTAC TGCTTCTCCAAAGATAGCGATCCTGCTAAGGATGTTCGTCAGCAGGCGGAAGCTGTGCTGGGCGTTTCCT TGGAGACCAGTAGTTCAGTTCATCTCGTAAGGAATGTGGCCCCTAACAAGGAGATGCTGTGCATCACCTT CCAGATTCCCACTGCTACACTCTACAGGAACCAAAGCCTCAGTCTACAGAATGACCAAGAACCACCTCTT AAACGGCAGAAGACAGGTGACCCGTTCTCAGGAGAACCACAGATCGCATCAGATTCTTAGTTTGAAATGT TTTCTCCATCTCCCCAGTAGACTTTATGTAAAGAAGTACAATTTGTAAGAGGTAAACTCTACCACACAGA TATTTGTTACTAAACACTTACGATTTGCTCTTATAAATGGAACATTACCAAAGTAAACTGAAATAAGAGG AACTTGGATATTGTATAGACTGATTTTTTGTTTCCATCCATTTTTCCTTTCTTTTTTATTATTTTTTGTG GCAGGATCTGTGTAACTCAGGGCTTAGAACTCTATCTTCCTGCTTCAGCCTCCCAGCTGTTGGCATTTCC AGCGTGCACCGCTGTTATTCAGCATAGTTTTAAAACAGAATCGGCATGGGCCAGTCAGACCAGTCAGATG GCTCACTGGGTGAAGGCACTTGCTGCCAAGCCTGATAGCCTGAGCCTGCCTAGGAACAACGTGGAAAGGA AGAAATCCTGAAAGTCTTCTTCTGACCTCATGCGTGCACCAAGGTACTCATGTATACACTTGCACAAAAT AAGCGAACAAATTAATAAACTTGGAAATTTATGTAAGGAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_029580
Insert Size 1506 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC012521, AAH12521
RefSeq Size 2080 bp
RefSeq ORF 1506 bp
Locus ID 76357
UniProt ID Q9D0C4
Cytogenetics 12 C3
Summary Involved in mitochondrial tRNA methylation (By similarity). Specifically methylates the N1 position of guanosine-37 in various tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniProtKB/Swiss-Prot Function
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"NM_029580" in other vectors (4)
SKU Description Size Price
MG208058 Trmt5 (tGFP-tagged) - Mouse TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (Trmt5) 10 ug
$667.00
MR208058 Trmt5 (Myc-DDK-tagged) - Mouse TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (Trmt5) 10 ug
$289.00 $467.00
MR208058L3 Lenti ORF clone of Trmt5 (Myc-DDK-tagged) - Mouse TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (Trmt5) 10 ug
$767.00
MR208058L4 Lenti ORF clone of Trmt5 (mGFP-tagged) - Mouse TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (Trmt5) 10 ug
$767.00

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