Suclg2 (NM_011507) Mouse Untagged Clone

SKU MC202753

Suclg2 (untagged) - Mouse succinate-Coenzyme A ligase, GDP-forming, beta subunit (Suclg2), nuclear gene encoding mitochondrial protein, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Suclg2
• Synonyms: AF171077; AW556404; D6Wsu120e; G-SCS; GTPSCS; SCS-betaG
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC080781 sequence for NM_011507; AAGAGGTAATCCGGTTCCTGGTTAAGATGGCGTCCCCGGTGGCCATCGCGGCGCAGGCTGGGAAGCTTCT GCGAGAGCGAGCGCTGCGGCCGCTCCTGGCGGTCAGGTCCCAGGCCGGTCACTTAACCCCCCGAAGATGG CTGAACCTGCAGGAGTACCAGAGCAAGAAGCTCATGTCAGAGCACGGAGTGAGAGTGCAGAGGTTCTTTG TTGCCAACACTGCTAAAGAGGCTCTGGAGGCAGCCAAGAGATTGAATGCAAAAGAAATTGTTTTAAAAGC CCAGATCTTAGCTGGAGGAAGAGGAAAAGGTGTCTTCAATAGCGGTTTGAAAGGAGGTGTTCATTTAACA AAAGACCCTAAAGTAGTGGGAGAGTTGGCTCAACAGATGATTGGTTATAATCTAGCAACGAAACAAACTC CAAAAGAAGGTGTGAAAGTTAACAAGGTGATGGTTGCTGAAGCCCTGGATATTTCTAGAGAAACATACTT GGCCATTCTAATGGACCGGTCCCATAATGGCCCTGTGATAGTGGGCAGTCCTCAGGGGGGCGTTGATATT GAGGAAGTGGCAGCTTCCAGTCCAGAACTCATCTTTAAGGAGCAGATTGACATCTTTGAAGGGATAAAGG ATAGCCAGGCCCAGCGCATGGCAGAAAATCCGGGCTTCCTTGGGTCTCTGAAAAACCAGGCTGCAGATCA GATTACAAAGCTGTACCATCTCTTCCTGAAGATTGATGCCACTCAGGTGGAAGTGAACCCCTTCGGTGAA ACTCCAGAAGGACAAGTTGTCTGTTTTGATGCCAAGATAAACTTTGATGACAATGCTGAGTTCCGACAAA AGGACATATTTGCTATGGACGACAAATCCGAGAATGAGCCCATTGAAAATGAAGCTGCCAGATACGATCT CAAGTATATAGGACTGGACGGGAACATTGCCTGCTTCGTGAATGGTGCTGGGCTGGCCATGGCTACCTGC GACATCATCTTCCTGAACGGAGGGAAGCCAGCGAACTTCTTGGACCTTGGAGGTGGTGTAAAGGAAGCCC AAGTCTATGAAGCATTCAAACTGCTCACGTCTGACCCCAAGGTGGAAGCCATTCTCGTCAATATCTTTGG TGGGATCGTCAACTGTGCCATCATTGCCAACGGAATCACCAAAGCCTGCCGGGAGCTGGAGCTCAAGGTG CCACTGGTAGTCCGGCTTGAAGGAACTAATGTGCAGGAGGCTCAGAATATCCTCAAGAGCAGCGGACTCC CCATCACGTCAGCTGTGGACCTGGAGGATGCAGCCAAGAAAGCTGTTGCCAGTGTGGCAAAGAAGTGATG CTCTCCCTGGCTCCCATGAAGAAGGGCATGTCCCTGACTACTGTGAAAGCAATTGTTTTCTCACTGGGAA GGGACACACACAAGGATCATCATGTGAAAAATATTAACTCTGAACTTTCTCAAATACGTTATCCAGAGTG CCTAAAGCTGAATTTATACTGTAGTCATTGTTAAAAAAAAAAATCACTCCTTACAATTTCACATCTTCTC TTGCCCATTCAGTAGCAGGGTCTACCAGCTCTGGGCCACAGTCAGTTTAGCCAAATAGTGTGCATACATA CAGTATTTAAAGCCAGATCTAGGTTCATTCACAAGGGCATTTATTCTACATTAGAAAATTTTACAGGACC CTCAAGTTCAGACAGGCTGATATTTAACATAGTGGCTTCCAGAAGGGTACAGCTAAGACACCTGAGCTTC TGTGACCAGTGGCCTCCACTGGCCCCTAATTCCAGTACATTAAGAAAGCCCATGAACATTTCTAAAGTGT GGCTTATATTGGGCATCCCGTGTTGATATATGTACATTGCACACATTTTACCCTGTGCCTTAATGTTGAA TCTACTGTTTGCCTCACACTAAGTTACTACCAGAAGCCTTAGTCATGGGTGTGAGGTGGAAGGTTTCATT TTGTGTCAGAGTTGCTGAATGTTAATCTATTTCATAAAGAGTCACACTAATGATCTAGCTGCTGGTAGCG GCCAGGCTTTCCCCTCTTGTCCCCTCCACCTCACATCTATCAGTTTGCCACAGAAAGATGAGAGATGCCA TTGGACAGAATGTGACCTTCCTAATGATGTCCTTTTTTGTGACAGGAATTTTCTTAACTTGATTTTTTTG ATTTAATTTTGATAAATTTATAGTCCTATACATTTTAAATAAAATTGTTTTTCAGTGCTAAAAAAAAAAA AAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011507
• Insert Size: 1302 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC080781, AAH80781
• RefSeq Size: 2243 bp
• RefSeq ORF: 1302 bp
• Locus ID: 20917
• UniProt ID: Q9Z2I8
• Cytogenetics: 6 44.63 cM
• Summary: GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longer isoform (1).