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Mmp2 (NM_008610) Mouse Untagged Clone

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SKU
MC202668
Mmp2 (untagged) - Mouse matrix metallopeptidase 2 (Mmp2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $617.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mmp2
Synonyms Clg; Clg4a; Ge; GelA; MMP-; MMP-2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC070430 sequence for NM_008610
CCAGCCGGCCACATCTGGCGTCTGCCCGCCCTTGTTTCCGCTGCATCCAGACTTCCCTGGTGGCTGGAGG CTCTGTGTGCATCCAGGAGTTTAGATATACAAAGGGATTGCCAGGACCTGCAAGCACCCGCGGCAGTGGT GTGTATTGGGACGTGGGACCCCGTTATGAGCTCCTGAGCCCCGAGAAGCAGAGGCAGTAGAGTAAGGGGA TCGCCGTGCAGGGCAGGCGCCAGCCGGGCGGACCCCAGGGCACAGCCAGAGACCTCAGGGTGACACGCGG AGCCCGGGAGCGCAACGATGGAGGCACGAGTGGCCTGGGGAGCGCTGGCCGGACCTCTGCGGGTTCTCTG CGTCCTGTGCTGCCTGTTGGGCCGCGCCATCGCTGCACCATCGCCCATCATCAAGTTCCCCGGCGATGTC GCCCCTAAAACAGACAAAGAGTTGGCAGTGCAATACCTGAACACTTTCTATGGCTGCCCCAAGGAGAGTT GCAACCTCTTTGTGCTGAAAGATACCCTCAAGAAGATGCAGAAGTTCTTTGGGCTGCCCCAGACAGGTGA CCTTGACCAGAACACCATCGAGACCATGCGGAAGCCAAGATGTGGCAACCCAGATGTGGCCAACTACAAC TTCTTCCCCCGCAAGCCCAAGTGGGACAAGAACCAGATCACATACAGGATCATTGGTTACACACCTGACC TGGACCCTGAAACCGTGGATGATGCTTTTGCTCGGGCCTTAAAAGTATGGAGCGACGTCACTCCGCTGCG CTTTTCTCGAATCCATGATGGGGAGGCTGACATCATGATCAACTTTGGACGCTGGGAGCATGGAGATGGA TACCCATTTGATGGCAAGGATGGACTCCTGGCACATGCCTTTGCCCCGGGCACTGGTGTTGGGGGAGATT CTCACTTTGATGATGATGAGCTGTGGACCCTGGGAGAAGGACAAGTGGTCCGCGTAAAGTATGGGAACGC TGATGGCGAGTACTGCAAGTTCCCCTTCCTGTTCAACGGTCGGGAATACAGCAGCTGTACAGACACTGGT CGCAGTGATGGCTTCCTCTGGTGCTCCACCACATACAACTTTGAGAAGGATGGCAAGTATGGCTTCTGCC CCCATGAAGCCTTGTTTACCATGGGTGGCAATGCAGATGGACAGCCCTGCAAGTTCCCGTTCCGCTTCCA GGGCACCTCCTACAACAGCTGTACCACCGAGGGCCGCACCGATGGCTACCGCTGGTGTGGCACCACCGAG GACTATGACCGGGATAAGAAGTATGGATTCTGTCCCGAGACCGCTATGTCCACTGTGGGTGGAAATTCAG AAGGTGCCCCATGTGTCTTCCCCTTCACTTTCCTGGGCAACAAGTATGAGAGCTGCACCAGCGCCGGCCG CAACGATGGCAAGGTGTGGTGTGCGACCACAACCAACTACGATGATGACCGGAAGTGGGGCTTCTGTCCT GACCAAGGATATAGCCTATTCCTCGTGGCAGCCCATGAGTTCGGCCATGCCATGGGGCTGGAACACTCTC AGGACCCTGGAGCTCTGATGGCCCCGATCTACACCTACACCAAGAACTTCCGATTATCCCATGATGACAT CAAGGGGATCCAGGAGCTCTATGGGCCCTCCCCCGATGCTGATACTGACACTGGTACTGGCCCCACACCA ACACTGGGACCTGTCACTCCGGAGATCTGCAAACAGGACATTGTCTTTGATGGCATCGCTCAGATCCGTG GTGAGATCTTCTTCTTCAAGGACCGGTTTATTTGGCGGACAGTGACACCACGTGACAAGCCCACAGGTCC CTTGCTGGTGGCCACATTCTGGCCTGAGCTCCCAGAAAAGATTGACGCTGTGTATGAGGCCCCACAGGAG GAGAAGGCTGTGTTCTTCGCAGGGAATGAGTACTGGGTCTATTCTGCTAGTACTCTGGAGCGAGGATACC CCAAGCCACTGACCAGCCTGGGGTTGCCCCCTGATGTCCAGCAAGTAGATGCTGCCTTTAACTGGAGTAA GAACAAGAAGACATACATCTTTGCAGGAGACAAGTTCTGGAGATACAATGAAGTGAAGAAGAAAATGGAC CCCGGTTTCCCTAAGCTCATCGCAGACTCCTGGAATGCCATCCCTGATAACCTGGATGCCGTCGTGGACC TGCAGGGTGGTGGTCATAGCTACTTCTTCAAGGGTGCTTATTACCTGAAGCTGGAGAACCAAAGTCTCAA GAGCGTGAAGTTTGGAAGCATCAAATCAGACTGGCTGGGCTGCTGAGCTGGCCCTGTTCCCACGGGCCCT ATCATCTTCATCGCTGCACACCAGGTGAAGGATGTGAAGCAGCCTGGCGGCTCTGTCCTCCTCTGTAGTT AACCAGCCTTCTCCTTCACCTGGTGACTTCAGATTTAAGAGGGTGGCTTCTTTTTGTGCCCAAAGAAAGG TGCTGACTGTACCCTCCCGGGTGCTGCTTCTCCTTCCTGCCCACCCTAGGGGATGCTTGGATATTTGCAA TGCAGCCCTCCTCTGGGCTGCCCTGGTGCTCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATGG CTTTCAGCACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCTGTTAACAGCCTGGCA TGGGGCAGTGGGGTACAGGTGTGCCAAGGTGGAAATCAGAGACACCTGGTTTCACCCTTTCTGCTGCCCA GACACCTGCACCACCTTAACTGTTGCTTTTGTATGCCCTTCGCTCGTTTCCTTCAACCTTTTCAGTTTTC CACTCCACTGCATTTCCTGCCCAAAGGACTCGGGTTGTCTGACATCGCTGCATGATGCATCTCAGCCCGC CTAGTGATGGTTCCCCTCCTCACTCTGTGCAGATCATGCCCAGTCACTTCCTCCACTGGATGGAGGAGAA CCAAGTCAGTGGCTTCCTGCTCAGCCTTCTTGCTTCTCCCTTTAACAGTTCCCCATGGGAAATGGCAAAC AAGTATAAATAAAGACACCCATTGAGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_008610
Insert Size 1989 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC070430, AAH70430
RefSeq Size 3070 bp
RefSeq ORF 1989 bp
Locus ID 17390
UniProt ID P33434
Cytogenetics 8 44.99 cM
Summary This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin and elastin. Mice lacking the encoded protein exhibit suppressed angiogenesis and attenuated features of human multicentric osteolysis with arthritis including abnormal skeletal and craniofacial development. provided by RefSeq, Feb 2016
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Documents
Resources
"NM_008610" in other vectors (4)
SKU Description Size Price
MG209857 Mmp2 (tGFP-tagged) - Mouse matrix metallopeptidase 2 (Mmp2) 10 ug
$817.00
MR209857 Mmp2 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 2 (Mmp2) 10 ug
$289.00 $617.00
MR209857L3 Lenti ORF clone of Mmp2 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 2 (Mmp2) 10 ug
$917.00
MR209857L4 Lenti ORF clone of Mmp2 (mGFP-tagged) - Mouse matrix metallopeptidase 2 (Mmp2) 10 ug
$917.00

file-alt Citations

Gelatinase A (MMP-2) promotes murine adipogenesis
Bauters, Scroyen, Van Hul et al
Biochim. Biophys. Acta (2015) 1850 (7), 1449-1456 DOI: 10.1016/j.bbagen.2015.04.003
Cited by 50
AI Summary: OriGene’s mouse Mmp2 expression vector (pCMV6-Mmp2) and empty plasmid (pCMV6) were used in this study to manipulate Mmp2 levels in 3T3-F442A preadipocytes, enabling the authors to assess the direct impact of MMP-2 on adipogenesis.
Snippet: differentiation medium was enriched with 10% BCS. The mouse Mmp2 expression vector (pCMV6-Mmp2) and empty plasmid (pCMV6) were obtained from OriGene (Sanbio, Uden, Netherlands). 3T3-F442A cells were transfected using ... Cite SaveSaving
Showing 1-1 of 1 papers.
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