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Tcf4 (NM_013685) Mouse Untagged Clone

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SKU
MC202522
Tcf4 (untagged) - Mouse transcription factor 4 (Tcf4), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $921.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tcf4
Synonyms 5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2; ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC043050 sequence for NM_013685
GTGATTTTGCTAAAATGCATCACCAACAGCGAATGGCTGCCTTAGGGACGGACAAAGAGCTGAGTGATTT ACTGGATTTCAGTGCGATGTTTTCGCCTCCTGTAAGCAGTGGGAAAAATGGACCAACTTCTTTGGCGAGT GGACATTTCACTGGCTCAAATGTAGAAGACAGAAGTAGCTCAGGGTCCTGGGGAACTGGAGGCCATCCAA GCCCGTCCAGGAACTATGGAGATGGGACTCCCTATGACCACATGACTAGCAGGGATCTTGGGTCACATGA CAATCTCTCTCCACCTTTTGTCAATTCCAGAATACAAAGTAAAACAGAAAGGGGCTCATACTCATCTTAT GGGAGAGAAAACGTTCAGGGTTGCCACCAGCAGAGTCTCCTCGGAGGGGACATGGATATGGGCAATCCAG GAACCCTTTCGCCCACCAAACCTGGCTCCCAGTACTATCAGTATTCAAGCAATAATGCCCGCCGGAGGCC TCTTCACAGTAGTGCCATGGAGGTACAGACAAAGAAAGTCCGAAAAGTTCCTCCGGGTTTGCCGTCTTCA GTCTACGCTCCTTCAGCCAGCACTGCCGACTACAACAGGGACTCGCCAGGCTATCCTTCCTCCAAGCCAG CAGCCAGCACTTTCCCTAGCTCCTTCTTCATGCAAGATGGCCATCACAGCAGCGACCCTTGGAGCTCCTC CAGCGGGATGAATCAGCCCGGCTACGGAGGGATGCTGGGCAATTCTTCTCATATCCCACAGTCCAGCAGC TACTGTAGCCTGCATCCACATGAACGTTTGAGCTATCCATCCCACTCCTCGGCAGACATCAACTCCAGTC TTCCTCCGATGTCCACGTTCCATCGTAGTGGCACAAACCATTACAGCACCTCTTCCTGCACACCCCCTGC CAACGGAACAGACAGTATAATGGCAAACAGAGGAACTGGGGCAGCAGGCAGCTCGCAGACTGGAGACGCT CTAGGGAAAGCCCTAGCTTCGATCTATTCTCCTGACCACACGAACAACAGCTTTTCCTCCAATCCTTCAA CTCCTGTGGGCTCCCCTCCTTCACTCTCAGCAGGCACAGCTGTTTGGTCTAGAAATGGAGGACAGGCCTC GTCATCTCCCAATTATGAAGGACCCTTGCACTCACTGCAAAGCCGAATCGAAGACCGTTTGGAAAGACTG GACGATGCGATTCATGTTCTCCGGAACCACGCAGTGGGCCCGTCCACAGCTGTGCCTGGTGGCCATGGGG ACATGCATGGGATCATGGGACCCTCCCACAACGGAGCGATGGGTAGCCTGGGCTCAGGGTACGGAACTAG TCTTCTCTCAGCCAACAGACACTCGCTCATGGTTGGGGCCCACCGTGAAGATGGCGTGGCTCTGAGAGGC AGCCATTCTCTCCTGCCAAACCAGGTTCCGGTCCCACAACTTCCGGTCCAGTCTGCAACTTCCCCTGACT TGAACCCACCCCAAGACCCTTACAGAGGGATGCCACCAGGCCTCCAGGGCCAGAGCGTGTCTTCTGGTAG CTCTGAGATCAAATCCGACGACGAGGGCGATGAGAACCTGCAAGACACAAAATCTTCTGAGGACAAGAAA TTAGATGACGACAAGAAGGATATCAAATCAATTACTAGGTCAAGATCTAGCAATAACGATGATGAGGACC TGACCCCAGAGCAGAAGGCTGAGCGCGAGAAGGAACGGAGGATGGCCAATAATGCCCGTGAGCGCCTGAG GGTCCGAGATATCAACGAGGCTTTCAAGGAGCTTGGCCGTATGGTGCAGCTCCACCTGAAGAGCGACAAG CCCCAGACCAAGCTCCTGATTCTCCACCAGGCCGTGGCTGTCATCCTCAGCCTGGAGCAGCAAGTTCGAG AAAGGAATCTGAACCCGAAAGCTGCCTGTCTGAAAAGAAGGGAGGAAGAGAAGGTGTCCTCAGAGCCTCC CCCACTCTCCTTGGCTGGCCCACACCCTGGGATGGGAGACGCAGCGAATCACATGGGACAGATGTGAAAA GGTCCAAGTTGCTACCTTGCTTCATTAAACAAGAGACCACTTCCTTAACAGCTGTATTACCCTAAACCCA CATAAACACTGCTCCTTAACCCCGTTTTTTTTTGTAATATAAGACAAGTCTGAGTAGTTATGAATCGCAG ACGCAAGAGGTTTCAGCATTCCCAATTATCAAAAAACAGAAAAACAAACAAAAAAATGAATGAAAGAAAG AAAGAAAGAAAAAAATGCAACTTGAGGGACGACTTCTTTAACATATCACTCTGAATGTGCGAAGCGGTAT GTACAGGCTGAGACACAGCCCAGAGACTGAATGGCAATCCTCCCACACTGTGGAGCAATGCATTTGTGCC TAAACTTCTTTTGGAAAAAAAAAATATAATTAATTTGTAAGTCTGAAAAAAATATTTAATTTAAAAAAAA ATTGTAAACTTGCAATAATGAAAAAGTGTACTTCTGAAGAAAACGACATGAACGTTTTTGTTGGTATTCA CGTCAGCTAGTGTTTCTAATTACCGGATATTGAATAGGGGAAGCCCGGCTGCCCTCGTAACAAAACCAGC AAACGTCCTGATGGCAACGAAGTGATGACATTAGCCATTCCTTAGGGTAGGAGGGACAGATGGATGTTAT AGACCTATGACAAATATATATATAAATATATATATAAATATATATTAAAAATTTAGTGACTATGGTAAGC TTTTGTTGATTTGTTTCAGACTTTTTTCTCCTGTAAAAAAATAGTACTGATTAACTTTTTTAAAAGAAAG ATTTTACTGTAAATATGGATTTTTTTTTTTGTCTGATTTTTGTCCCTTCCCCCGGTTTGTTATCGTAACC TGTAGTGCCAACTCTGCTTCCGGAGGGGCAGTGCAGGACGAAATGCTGACCCTGAAGTTGCTTCTCATTC ACAAATAGTAAAAAGTTGTTTCTCCAGTCTTTTGGGAACACAGGACTTAAAAGTCACATCATGTGTAGGA ATTACATGCAGCATTGCCCGGGCGAGGCAAAAAGCGTTTGTCTGGCTTGTGGCGCTGCCCTTGTTACCCT CCCCTGGGATTTTCAGAGGTACACGGTTAGAATGCTACAATGTTACCACTGTGCCTTCCAATGTTTATAT CATCGGAAACATAACATAATCAAAGTGGCTGTGATTTAACAAAAAAAACGATTCAAGTGTTACCTACCTG TGTAGCCGAAGTAGTGTGCAGTGACCGAGACGTTTCTGAATACATGGTCAGATTTTTTTTGGAAAAAATA CAAAAATTAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_013685
Insert Size 2013 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC043050, AAH43050
RefSeq Size 3318 bp
RefSeq ORF 2013 bp
Locus ID 21413
UniProt ID Q60722
Cytogenetics 18 E2
Summary Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform a).
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"NM_013685" in other vectors (4)
SKU Description Size Price
MG209915 Tcf4 (tGFP-tagged) - Mouse transcription factor 4 (Tcf4) 10 ug
$1,120.00
MR209915 Tcf4 (Myc-DDK-tagged) - Mouse transcription factor 4 (Tcf4), transcript variant 1 10 ug
$920.00
MR209915L3 Lenti ORF clone of Tcf4 (Myc-DDK-tagged) - Mouse transcription factor 4 (Tcf4), transcript variant 1 10 ug
$1,220.00
MR209915L4 Lenti ORF clone of Tcf4 (mGFP-tagged) - Mouse transcription factor 4 (Tcf4), transcript variant 1 10 ug
$1,220.00

file-alt Citations

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