Smpd3 (NM_021491) Mouse Untagged Clone

SKU MC202307

Smpd3 (untagged) - Mouse sphingomyelin phosphodiesterase 3, neutral (Smpd3), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Smpd3
• Synonyms: 4631433G07Rik; AI427456; AW537966; fro; nSMase2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046980 sequence for NM_021491; GGCTCATCACCCTTTCAGGTCCTGATTGCTTGGACCTCCTGTCTCCAGAGGCAAGGACACAAGGCCCAAG AGGCCCCTCCTCAGGTTTTCAGCAGACTCCGGTGTCCCAGCATGGCCCCTTGAAGCGAACCATGACTGTC TGTGGGACACTCGCCACTGATCATCCAGAGTCACATCTCACCAGGAGGTGACCTCTCCTCCATCCTCCAC CTGCCCCCTGCCTGGGACAGTGCTGCGGCTGCCACGGACACCATGTAGTAGAGCCGGCTGCGGCGCCCAG TGAGCTGCAATGGTTTTGTACACGACCCCCTTTCCTAATAGCTGTCTGTCTGCCCTCCACGCCGTGTCCT GGGCCCTCATCTTCCCATGTTACTGGCTGGTGGACCGGCTGTTGGCCTCTTTCATACCCACCACCTATGA GAAGCGCCAGAGGGCAGATGACCCCTGCTGCCTGCAGCTGTTCTGCACGGTCCTCTTCACGCCAGTCTAC CTGGCTCTGCTTGTGGCTGCACTGCCCTTTGCCTTTCTCGGGTTCATCTTCTGGTCTCCACTGCAGTCTG CCCGCCGGCCCTACTCCTACTCCCGGCTAGAAGACAAGAACCCAGCTGGTGGGGCAGCCCTGCTTAGTGA ATGGAAGGGCACGGGGGCAGGCAAAAGCTTTTGCTTCGCCACGGCCAATGTCTGCCTCCTCCCCGACTCA CTCGCGAGGCTCAATAATGTGTTCAACACTCAAGCTCGGGCCAAAGAGATTGGGCAGAGAATCCGCAATG GGGCCGCCAGGCCTCAGATCAAGATCTACATCGATTCTCCCACCAACACCTCCATCAGTGCAGCCAGCTT CAGCAGCCTGGTGTCGCCACAGGGTGGTGATGGATCCAGAGCTGTTCCTGGGAGCATTAAGAGGACAGCC TCTGTGGAATACAAGGGGGATGGTGGGCGTCACCCCAGCGACGAGGCTGCCAACGGCCCAGCCTCTGGAG AACAGGCTGACGGAAGCCTTGAGGACAGCTGCATTGTGCGAATTGGTGGCGAGGAAGGAGGTCGGCCACA AGAAGCTGATGACCCTGCTGCCGGGAGCCAAGCCAGGAATGGGGCTGGTGGGACCCCAAAGGGCCAGACA CCCAACCACAATCAGCGAGATGGGGATTCTGGGAGCCTGGGCAGCCCCTCAGCCTCCAGGGAGTCCTTGG TGAAGGCACGGGCTGGGCAAGACAGTGGTGGCAGCGGAGAGCCAGGGGCTAACAGCAAGCTGCTGTATAA GACCTCTGTGGTGAAGAAGGCAGCAGCCCGCAGGAGGCGCCACCCTGATGAAGCCTTTGACCACGAGGTC TCAGCCTTCTTCCCGGCCAATCTGGACTTCCTGTGCCTGCAGGAGGTGTTTGACAAGCGTGCGGCTGCTA AGTTGAAAGAGCAGCTACACGGCTACTTCGAGTACATCCTGTATGATGTTGGGGTCTACGGTTGTCATGG TTGCTGCAATTTCAAATGTCTCAACAGCGGTCTCTTCTTCGCCAGCCGCTACCCTGTCATGGACGTGGCC TATCACTGTTACCCCAATGGGTGCAGCTTCGATGCCCTGGCCTCTAAGGGAGCTCTGTTTCTCAAGGTGC AGGTGGGAAGCACACCTCAGGACCAAAGAATTGTTGGGTACATCGCCTGCACACACCTGCATGCCCCACC AGAGGACAGTGCCGTCCGGTGTGAGCAGCTGGACCTGCTTCAGGACTGGCTGGCTGATTTCCGAAAATCT ACCTCCTCGACCAGCACAGCCAACCCCGAGGAGCTGGTGGTGTTTGACGTCATCTGTGGAGATTTGAACT TTGATAACTGCTCTTCTGATGACAAACTGGAGCAGCAGCACTCGCTCTTTACTCGCTACAAAGACCCCTG CCGCCTTGGGCCCGGGGAGGAGAAGCCTTGGGCCATTGGCACCCTGCTGGACACAAACGGTCTCTATGAT GAGGATGTGTGTACCCCGGACAACCTTCAAAAGGTGTTGGAGAGCGAGGAAGGCCGCAGGGAGTATCTGG CCTTCCCCACCAGCAAGAGCCCAGGTGCTGGGCAGAAGGGCCGGAAGGATCTGCTGAAGGGCAATGGCCG CCGCATCGACTACATGCTGCACGCCGAGGAGGGGCTGTGCCCTGACTGGAAGGCTGAGGTAGAAGAATTC AGTTTTATCACCCAGCTGTCCGGCCTGACCGACCACCTCCCCGTGGCCATGCGGCTGATGGTGTCTGCAG GGGAAGAGGAGGCATAGACCAGCCATGCCACGGTCTCGAGCAGTAGGGCCTCTGCCGGCCCTTCAAGCCA CAGCCCGTGCCTGGGCTGTGTGCCCTCCATTGAGCACCCAGTGCTGGGGGAAGGAGGGCAGGGGACAGGG AAGGACGAGCCCATCAATCCCCTGCAAGCCTGGGAACCAGGCTGCTCTGTGCCTCTGGGCCAACTCTGCG GACAGAGGACTTGGGCCAGCCTGGGAGCCCCCGGCTGCCTGACCAGTGCCATTCTTTCAGTCACGATTTT CTACAGAAATACACAGCACAACCTAGTTTGTAACCCAAGGGTTAGTACGAGGACCGGGTTTCTGTACTCT TTGTATCTCTTCTCAAGCTTTGTCCAGGGTTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021491
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC046980, AAH46980
• RefSeq Size: 2654 bp
• RefSeq ORF: 1968 bp
• Locus ID: 58994
• UniProt ID: Q9JJY3
• Cytogenetics: 8 D3
• Summary: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Acts as a regulator of postnatal development and participates in bone and dentin mineralization. Overexpression enhances cell death, suggesting that it may be involved in apoptosis control.[UniProtKB/Swiss-Prot Function]