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Mettl14 (NM_201638) Mouse Untagged Clone

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SKU
MC202029
Mettl14 (untagged) - Mouse methyltransferase like 14 (Mettl14), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mettl14
Synonyms G430022H21Rik; mKIAA1627
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC052204 sequence for NM_201638
GTGAAAGTCTCCGGGTCAGAATCTCTGCGGGGCGGGACCCGGTGTTTCCTGGTTTGGCAGGTGGATTTGC ATTTTGGCGGGCGCGGAGTCCGTCGGGGTGGTGAGCAGCCGAAGCTGGGGCATGGATAGCCGCCTGCAGG AGATCCGGGAGCGGCAGAAGTTACGGCGGCAGCTCCTAGCTCAGCAGTTGGGAGCTGAGAGTGCGGATAG CATTGGTGCTGTGTTAAATAGCAAAGATGAACAGAGGGAGATTGCTGAAACCAGAGAAACCTGCAGGGCT TCCTATGATACATCTGCTCCAAACTCAAAACGGAAGTGTCTGGATGAAGGAGAGACTGATGAAGACAAAG TAGAAGAATATAAGGATGAACTGGAAATGCAGCAGGAGGAAGAGAATTTGCCATATGAAGAAGAGATTTA CAAAGATTCCAGTACCTTTCTTAAGGGAACGCAGAGCTTAAATCCCCATAATGATTACTGCCAACATTTT GTAGACACTGGACACAGACCTCAGAATTTCATCAGGGATGTAGGTTTAGCTGACAGATTTGAAGAATACC CTAAACTTAGGGAACTCATCAGACTAAAGGATGAGCTAATAGCTAAGTCAAACACTCCTCCCATGTACTT ACAAGCAGACATAGAAGCCTTTGACATCAGAGAATTGACACCCAAATTTGATGTGATTCTCCTGGAGCCC CCTCTGGAAGAATACTATAGAGAGACTGGCATCACTGCGAATGAGAAATGCTGGACCTGGGATGATATTA TGAAGTTAGAAATCGATGAGATTGCAGCACCTCGGTCATTTATATTTCTCTGGTGCGGTTCTGGGGAAGG ATTGGACCTTGGGAGAGTATGCTTGCGAAAGTGGGGTTACAGAAGATGTGAAGATATTTGTTGGATTAAA ACCAATAAAAACAATCCTGGAAAGACAAAGACTCTAGATCCAAAGGCAGTTTTCCAGAGAACAAAGGAGC ATTGCCTGATGGGGATCAAAGGAACCGTGAAGCGAAGCACAGACGGGGACTTCATTCATGCTAATGTTGA CATTGACTTAATTATCACAGAAGAACCTGAGATTGGCAATATAGAAAAACCAGTAGAAATTTTTCATATA ATAGAACATTTTTGTCTTGGTAGAAGACGCCTTCATCTCTTTGAGAGAGATAGCACTATCAGGCCAGGCT GGCTCACAGTTGGACCAACGCTTACAAACAGTAACTACAATGCAGAAACATATGCATCGTATTTCAGTGC CCCCAACTCATACTTGACCGGATGTACAGAGGAAATCGAGAGGCTTCGACCGAAGTCACCTCCTCCCAAG TCCAAGTCTGACCGTGGGGGTGGAGCTCCCAGAGGTGGAGGAAGGGGGGGAACATCTGCTGGCCGTGGTC GGGAAAGAAACCGATCCAATTTCCGAGGAGAGAGAGGTGGCTTTAGGGGGGGCCGTGGAGGCACGCACAG AGGCGGCTTTACTCCTCGGTAGCTCTTACACTTGCATCTTTATCACAGTGGAGACTTGCTTCAGAGCTCA CTCCCAGCTTGTACTTTGCTTTAGTTTCTCATGCTGCCAGAAAGATCTTAGCCACCCCACCCCACCCCAG GGACTGGCAGTTGTCACAGACTGAGCTTTTGCTCAGGGTGGGTGAGTCACGTGTGCGCAACACCCCGACC TGACTTGACTGATTCCAGTCAAGGCTTTGGCTTCCTGGAAATGGCTGCGCAGAGGCTTCGGCATCTCAGA GGAGTGGCAGGAGTGGGTGCTGTGTTCCCTGTCATGGCTGCACGGCAGCAGGAGGCTGGGAACCCGGGTA GAACAGAATTAGAGAGGTGGTCACTGTGTTGGGCCTTTAATGTTTTCTTGTTGAAACACTAAAAAGAGAA GAGTTTTTCCCCTTACTTTTAAATTCTAAAAAATAACATGAAGTATCATTTGAGTTCCCAGAACTCGGGA GGGGAAATGAAATCTCGTTCTTCCAGATCAGAATCTGAGGTGGTTGCAGTCGTCAGGGTAGGTAACGGGA GAGGCAGGCAGAGCATGGGATATTTCAAACAAAACCAAGCTTATCATAATGTAAATTAGCGGCTACAGAT GGCTCCTGACCATGTGTCTTCAGAAGCAAGACAAGGCATGTATGTCTCCAGGTCGGAGTGTGAACCTGAT CTTGGGCTAACCTAAGATAATTCAGATTTCTTAGCAAAGGCAAAAACAGATTTGGGGAGGGGCCGGTTTA AACGGTTGAGAAAATAAAATCTTGATGATGAGGAAATGACAAACATCTTTAAAGTTGTTACTAAAGAAAA TTTAGGTAAAAGCAATTCCAGAAAACCCTTACGAATACAGCGTGGAAGAGGACCTGCAGTGTCCTTTGAT TAGAACTATGTGGTGCAGAAGTCGCCGCAGGGCTGATGGGCAGATCTCTTGGCTGTGGGAAGCTTCGTGC TGTTGCCTTTCTACACCTTAAGTCCTATTTGATGGATTTGGATATGCCAGATGAGATAATGCCAATATTG TTCTGGGTAACTCCTTTTTTATTTTGCTATAACTTTTCTAATAAAAATTTAAACCTTTGAAAAAAAAAAA AAAAA
Restriction Sites RsrII-NotI |
ACCN NM_201638
Insert Size 1371 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052204, AAH52204
RefSeq Size 2595 bp
RefSeq ORF 1371 bp
Locus ID 210529
UniProt ID Q3UIK4
Cytogenetics 3 G1
Summary The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24394384, PubMed:28965759). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (By similarity). N6-methyladenosine (m6A), which takes place at the 5'-AGGAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (By similarity). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (PubMed:24394384). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (PubMed:24394384). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (PubMed:28914256). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (PubMed:28965759).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_201638" in other vectors (4)
SKU Description Size Price
MG207291 Mettl14 (tGFP-tagged) - Mouse RIKEN cDNA G430022H21 gene (G430022H21Rik) 10 ug
$657.00
MR207291 Mettl14 (Myc-DDK-tagged) - Mouse methyltransferase like 14 (Mettl14) 10 ug
$289.00 $457.00
MR207291L3 Lenti ORF clone of Mettl14 (Myc-DDK-tagged) - Mouse methyltransferase like 14 (Mettl14) 10 ug
$757.00
MR207291L4 Lenti ORF clone of Mettl14 (mGFP-tagged) - Mouse methyltransferase like 14 (Mettl14) 10 ug
$757.00

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