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Polr1d (NM_181730) Mouse Untagged Clone

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SKU
MC201795
Polr1d (untagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $150.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Polr1d
Synonyms 16kD; 1110003G10Rik; AC19; mRP; RPA16; Rpo; Rpo1-3
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC024394 sequence for NM_181730
CCCGCACCTTTCGCGACATGGGGAAGAGCCTCCATTTCGCCAGCGCCACCTGAGGATTTCCTGGAGCCGC CACCCCCACCTCCCGCGATGGAAGACGATCAGGAGCTGGAGAGGAAAGCCATAGAGGAATTGCTAAAGGA GGCAAAGCGTGGGAAAACCAGAGCGGAGACAATGGGGCCGATGGGCTGGATGAAGTGTCCTCTTGCTGGT ACAAATAAAAGATTCCTAATTAACACAATTAAGAACACACTGCCCTCCCATAAAGAGCAAGACCATGAAC AAAAAGAGGGCAGTAAGGAGCCTGGCAAAAGCCAAGACCAGAAGGAAGCAAGTGGGAAGAAATACCGAAG TCACTCGTACAAGCGCAGCCTTCACTCGTCCCGGGGCTCCGCAGGCTGCTCTCCTCCACGGAAGCGGACC TCCCGGACCTCCGGGGACAAGTGCGACCCACGGCCCAGCAGGCGATGAGACAGCCGCAGGGCTAGCCCAG CCCAGCACTGCACCTGCCACTGAGCGAAGCAAGGAAGACAGGGAGGTGGCCGGCTGGCACCTGAGAGTGA CTTTCTCAGTGGGTCCTTTGTTTTAAGGAGGCTGTTGGTACTGTGTGGTTGTTCTTGTTGCTGTTGTTGT TGTTGTTAACGGGAGAAGAAAACTTTACTAAAACAAATCTTAACTACCTTATCCTGAAGGTGTCGCGGTG AGAGCAAGCTGACTCGGCATGGCCCCCGCATATCCGTGGTGTCTTTATCCTACCTTACTTGGAAGTTCGT TTCAAAACAGGCAGTTTTAGAATTTGAGATTTTGAGATTGTTTAAAAACTCCTAGTTTTTTGATGTACTG CTCTTTTGTGGTCATTTCCCAGAATTTCTCAAAAAAATCAATAAAATACTTAGAAACGCGAAAAAAAAAA AAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_181730
Insert Size 381 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC024394, AAH24394
RefSeq Size 919 bp
RefSeq ORF 381 bp
Locus ID 20018
Cytogenetics 5 G3
Summary This gene encodes an RNA polymerase subunit that is a component of both the RNA polymerase I and RNA polymerase III complexes. RNA polymerase I is associated with transcription of pre-ribosomal RNAs, while RNA polymerase III is associated with transcription of small RNAs. Pseudogenes of this gene have been defined on chromosomes 4 and 6. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2013
Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct and shorter C-terminus, compared to isoform 1.
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"NM_181730" in other vectors (4)
SKU Description Size Price
MG200671 Polr1d (tGFP-tagged) - Mouse RNA polymerase 1-3 (Rpo1-3), transcript variant 2 10 ug
$350.00
MR200671 Polr1d (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2 10 ug
$289.00
MR200671L3 Lenti ORF clone of Polr1d (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2 10 ug
$450.00
MR200671L4 Lenti ORF clone of Polr1d (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2 10 ug
$450.00

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