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Bgn (NM_007542) Mouse Untagged Clone

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SKU
MC201242
Bgn (untagged) - Mouse biglycan (Bgn), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Bgn
Synonyms BG; DSPG1; PG-S1; PGI; S; SLRR1A
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC019502 sequence for NM_007542
CGGACGCGTGGGCGAACTGCCCAGGAGCGAGCAGCTGCTCCCGGTTGGCCCTGACGGACAGACAAACCGA CAGCCTGACAACCTAGTCCACCAACTAAGCAGCCTGCACCTGGCTGCTTGTCCCTCCCCAGGAACATTGA CCATGTGTCCCCTGTGGCTACTCACCTTGCTGCTGGCCCTGAGCCAGGCCTTGCCCTTTGAGCAGAAGGG TTTCTGGGACTTCACCTTGGATGATGGGCTGCTCATGATGAATGATGAGGAGGCTTCAGGTTCAGACACC ACTTCAGGTGTCCCCGACCTGGACTCTGTCACACCTACCTTCAGTGCCATGTGTCCTTTCGGTTGCCACT GCCACCTGCGGGTTGTTCAGTGCTCTGACTTGGGTCTGAAGACTGTGCCCAAGGAGATCTCACCTGACAC CACACTGCTAGACCTGCAGAACAATGACATTTCTGAGCTTCGCAAGGATGACTTCAAAGGCCTCCAGCAC CTCTACGCCCTGGTCTTGGTAAACAATAAGATCTCCAAGATCCATGAGAAGGCCTTTAGCCCTCTGCGGA AGCTGCAAAAACTCTACATCTCCAAGAACCACCTGGTGGAGATTCCTCCCAACCTGCCCAGCTCCCTGGT AGAACTACGAATCCATGACAACCGTATCCGCAAAGTGCCCAAGGGCGTGTTCAGCGGGCTCCGGAACATG AACTGCATTGAGATGGGCGGGAATCCCCTGGAGAACAGTGGCTTTGAACCAGGAGCCTTTGATGGCCTGA AGCTCAATTACCTGCGCATCTCAGAGGCCAAGCTCACTGGCATCCCCAAAGATCTCCCTGAGACCCTGAA CGAACTTCACCTGGACCACAACAAAATCCAGGCTATTGAGTTGGAGGACCTACTTCGATACTCCAAGCTG TACAGGTTGGGCTTAGGTCACAATCAGATTCGGATGATTGAGAATGGGAGCCTGAGTTTTCTGCCTACCC TGAGGGAACTTCACTTGGACAACAACAAGCTGTCCCGGGTGCCTGCTGGCCTCCCAGATCTCAAGCTCCT CCAGGTTGTCTATCTGCACTCCAACAACATCACCAAGGTGGGCATCAATGACTTCTGTCCTATGGGCTTC GGAGTCAAGAGGGCCTACTATAATGGCATCAGCCTCTTCAACAACCCTGTGCCCTACTGGGAAGTGCAGC CTGCCACCTTCCGCTGCGTTACTGACCGCCTGGCCATCCAATTTGGAAATTATAAGAAGTAGAGGCAGTG GTTGCCACCATGGTGGCCTTGGTGAGAGTCTCTGAGGAACATAGCCAGATGAAGAAGCAACACCTTTGTT CCCCAATATTAACTCACTGCCCCACCACAGCTTCCCCCTGACTCCTAAGCATGCATATATGCACATGGCC TGGCCCTCTCACCCATTCCCCTCAACCTTTGAAATTTAACATTCATCAACCATGTCCACTCAGAGACTCC CTATAAATCTTTCTTCTTGCTCATCCTGAAACTCAGATGTTTTTGGCAAGAGGGGCTAGGAAAGATGGAT AGAGCACACTGCCACCGCCATTGTTCCATCCAGGCATGTGTTCCTCCTCTTCCTTGCTCATGTCTGACTT CCAGCTCTCCTGGGCTCTGCTTGCTGCCCTTATCCTCTGGTGTTCTCTCTTCAACAAGTTCACTACCTGT CAATCCCAGCTACAACCTGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACCCTGTTATGCTTCCT GACACTTTTCTTCCTTCTGGAGTTATTGACCTGTCCCCTTCCATCTCTGGACCTAGGTCATATTTCTCCA TCTTTGTCTCTTTCTGTATCTCCTTGCCTATATCTCTGTCTGTCTCTATTTCTGTCTCTCTGTCTCTGTG TATCTCTCTATCTTTGTATCTGTCTCTCTCTGACAACACACACACACACACACACACACACACACACACA CACACGGATCATCTGCCCCAGGCTGCTTTCTGCTTCACAGGTCTCTAGCCAGTCCCTCCACAAACAAATA TGGGGCAACTATCTTCCTGATTGCCCTACCCAGAACTTGACCCCCCAACCCTGGAGGAAGCTGGAAGGTG GAGGCCCAGAATCCTGTCCATTTTGTCCAGGAAAGGGTTCATACTCTGCTATCAAGACGAGGATCAAGGA GCTTCCTAGCCCCTGGAGAGGCTCAGCAGGCCATCAGAGCCGCCAGAACCAGTTTGCATTGGCCCCTGCT CTCTCCCCAAGATGGCTAGGTCCCCTCCCTCACCCCTGGGTCCCTGATGTGGTAGGAGGTGATGGTCAGT TGCACCCAGCAAGAGGGAGTGCTGCTTATGAGGTCAGTTGTCTCTCAATTAAAGAAACACTGTGCAAAAA AAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_007542
Insert Size 1110 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019502, AAH19502
RefSeq Size 2390 bp
RefSeq ORF 1110 bp
Locus ID 12111
UniProt ID P28653
Cytogenetics X 37.33 cM
Summary This gene encodes a small, leucine-rich repeat proteoglycan that plays important roles in bone mineralization and connective tissue metabolism. The encoded preproprotein undergoes post-translational processing during which chondroitin sulfate or dermatan sulfate chains are attached before incorporation into the extracellular matrix. Mice lacking the encoded protein exhibit reduced growth rate and acquire diminished bone mass progressively with age. provided by RefSeq, Oct 2015
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Documents
Resources
"NM_007542" in other vectors (4)
SKU Description Size Price
MG205682 Bgn (tGFP-tagged) - Mouse biglycan (Bgn) 10 ug
$657.00
MR205682 Bgn (Myc-DDK-tagged) - Mouse biglycan (Bgn) 10 ug
$289.00 $457.00
MR205682L3 Lenti ORF clone of Bgn (Myc-DDK-tagged) - Mouse biglycan (Bgn) 10 ug
$757.00
MR205682L4 Lenti ORF clone of Bgn (mGFP-tagged) - Mouse biglycan (Bgn) 10 ug
$757.00

file-alt Citations

Chondroitin sulfate proteoglycan promotes APRIL‐induced tumor cell proliferation
Nadanaka, Koike, Kitagawa
Proteoglycan Research (2024) 2 (1) DOI: 10.1002/pgr2.15
Snippet: … Understanding the;Full-length mouse biglycan (BGN) gene (#MC201242) was purchased from OriGene. Cloning of the full-length mouse Bgn into the pcDNA3.1(+) mammalian expression vector (Thermo Fisher Scientific) was performed using the In-Fusion HD Cloning Kit (Takara Bio USA Inc.) with the following primers: forward primer, 5′-ACCCAAGCTGGCTAGCACCATGTGTCCCCTGTGGCT-3′ (underline: NheI recognition site;bold: initiation codon);reverse primer
Aberrant glycosaminoglycan biosynthesis by tumor suppressor EXTL2 deficiency promotes liver inflammation and tumorigenesis through Toll-like 4 receptor signaling
Nadanaka, Hashiguchi, Kitagawa
FASEB J. (2020)
AI Summary: OriGene contributed to this study by providing the full-length mouse BGN gene (#MC201242), which was used to construct a BGN expression vector for investigating the role of glycosaminoglycan biosynthesis in liver inflammation and tumorigenesis.
Snippet: eluent. The flow‐through fractions were collected and evaporated to dryness. 2.9 Construction of the BGN expression vector. A full‐length gene of mouse BGN (#MC201242) was purchased from OriGene (Rockville, MD). Cloning
Showing 1-2 of 2 papers.
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