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Gfpt1 (NM_013528) Mouse Untagged Clone

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SKU
MC201036
Gfpt1 (untagged) - Mouse glutamine fructose-6-phosphate transaminase 1 (Gfpt1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $625.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gfpt1
Synonyms 2810423A18Rik; AI324119; AI449986; GFA; GFAT; GFAT1; GFAT1m; Gfpt
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC010516 sequence for NM_013528
CGGACGCGTGGGCTCATTGCCACCACCCCGGCCCGAGCTCACCCTCGCTTCTGAAGCTCTCCGCGCGCCC GACAGCTCAGCCCTCGCCCGTGACCAACATCATGTGCGGTATATTTGCTTATTTAAATTACCATGTTCCT CGAACAAGACGAGAAATCTTGGAGACACTAATCAAAGGCCTTCAGAGACTGGAATACAGAGGATATGATT CTGCTGGTGTGGGACTTGACGGAGGCAATGACAAAGACTGGGAAGCCAACGCCTGCAAAATCCAGCTCAT TAAGAAGAAAGGAAAAGTTAAGGCACTGGATGAAGAAGTTCACAAACAACAAGATATGGACTTGGATATA GAATTTGATGTGCATCTTGGAATAGCTCATACCCGTTGGGCGACAACAGGAGAACCCAATCCTGTCAATA GTCACCCCCAGCGCTCTGATAAAAATAATGAATTCATTGTTATTCATAATGGAATCATCACCAACTACAA AGACTTGAAAAAGTTTCTGGAAAGCAAAGGCTATGACTTTGAATCTGAAACAGACACAGAAACCATTGCC AAGCTCGTCAAGTACATGTATGACAACTGGGAGAGCCAGGACGTCAGTTTTACCACCTTGGTGGAGAGAG TTATCCAACAATTGGAAGGCGCCTTTGCTCTTGTGTTTAAAAGTGTCCATTTTCCCGGGCAAGCAGTTGG CACAAGGCGAGGTAGCCCTCTCTTGATTGGTGTGCGGAGTGAACATAAGCTTTCTACAGATCACATTCCG ATTCTGTACAGAACAGGCAAAGACAAGAAAGGAAGCTGCGGTCTTTCCCGTGTGGACAGCACGACATGCC TGTTCCCTGTTGAGGAAAAGGCAGTTGAATATTACTTTGCTTCTGATGCAAGTGCCGTGATAGAGCACAC CAATCGTGTCATCTTTCTGGAAGATGATGATGTTGCAGCAGTGGTGGATGGCCGTCTCTCTATCCACCGA ATTAAACGAACTGCAGGAGACCATCCTGGCCGAGCTGTGCAAACTCTCCAGATGGAGCTCCAGCAGATCA TGAAGGGCAACTTTAGTTCATTTATGCAGAAGGAAATTTTTGAGCAGCCAGAATCTGTTGTGAACACAAT GAGAGGAAGAGTCAATTTTGACGACTACACTGTGAATTTGGGAGGTTTGAAAGATCACATTAAGGAGATC CAGCGGTGTCGGCGGTTGATTCTTATTGCTTGTGGCACAAGTTACCACGCTGGTGTGGCAACCCGTCAGG TCCTGGAGGAGCTGACCGAGCTGCCCGTGATGGTGGAGCTTGCCAGTGACTTCTTGGATAGAAACACTCC AGTCTTTCGAGATGATGTTTGCTTTTTCATTAGTCAATCAGGCGAGACAGCTGACACCCTGATGGGACTT CGTTACTGTAAGGAGAGAGGAGCCTTAACTGTGGGGATCACAAATACAGTCGGCAGTTCTATATCAAGGG AGACAGATTGCGGGGTTCATATTAATGCTGGTCCTGAGATTGGCGTGGCCAGTACAAAGGCATACACCAG CCAGTTTGTGTCCCTCGTGATGTTTGCTCTCATGATGTGTGATGACAGGATCTCCATGCAAGAGAGACGC AAAGAGATCATGCTCGGACTGAAGCGACTGCCGGACTTGATTAAGGAAGTGCTGAGCATGGATGATGAAA TCCAGAAGCTGGCGACGGAGCTTTACCACCAGAAGTCGGTCCTGATAATGGGGCGGGGCTACCATTATGC TACATGCCTTGAAGGGGCTCTGAAAATCAAGGAGATTACTTATATGCATTCGGAAGGCATCCTTGCTGGT GAGCTCAAGCACGGCCCTCTGGCCTTGGTGGACAAGTTGATGCCTGTCATCATGATCATCATGCGAGACC ACACTTATGCCAAGTGCCAGAACGCTCTTCAGCAGGTGGTTGCACGGCAGGGGCGTCCAGTCGTGATCTG TGATAAGGAGGATACTGAAACCATTAAGAATACAAAAAGGACAATCAAGGTGCCCCACTCAGTGGACTGC TTGCAGGGCATTCTCAGTGTGATTCCCCTGCAGCTGCTGGCTTTCCACCTGGCTGTGCTGAGAGGCTACG ATGTTGATTTTCCACGGAATCTTGCCAAATCTGTAACAGTAGAGTAACAGACACCTGAAACTTAAGACAG TTAAGCAACACGAGATACCTTTTGTATTTAAATTTTTGATTTAAACTATTGCCTCCTGAAAGCCTTTTTT TAGTAAATCTTATTTATATATCAGTTATAATTATATCAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_013528
Insert Size 2046 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC010516, AAH10516
RefSeq Size 2302 bp
RefSeq ORF 2046 bp
Locus ID 14583
UniProt ID P47856
Cytogenetics 6 37.81 cM
Summary Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013528" in other vectors (4)
SKU Description Size Price
MG210002 Gfpt1 (tGFP-tagged) - Mouse glutamine fructose-6-phosphate transaminase 1 (Gfpt1) 10 ug
$825.00
MR210002 Gfpt1 (Myc-DDK-tagged) - Mouse glutamine fructose-6-phosphate transaminase 1 (Gfpt1) 10 ug
$589.00 $625.00
MR210002L3 Lenti ORF clone of Gfpt1 (Myc-DDK-tagged) - Mouse glutamine fructose-6-phosphate transaminase 1 (Gfpt1) 10 ug
$925.00
MR210002L4 Lenti ORF clone of Gfpt1 (mGFP-tagged) - Mouse glutamine fructose-6-phosphate transaminase 1 (Gfpt1) 10 ug
$925.00

file-alt Citations

Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle
Weyrauch, McMillin, Witczak
Int J Mol Sci (2020) 21 (13) DOI: 10.3390/ijms21134715
Cited by 21
AI Summary: OriGene products—untagged mouse G6PDx (cat#MC205271) and untagged mouse GFPT1 (cat#MC201036) expression vectors—were used to overexpress these genes in mouse muscle, enabling the study of their roles in glucose metabolism under mechanical overload and insulin resistance.
Snippet: x or GFPT1 mouse muscle overexpression samples that were generated by transfecting the tibialis anterior muscles of male CD-1 mice (8 weeks old) with empty vector, untagged mouse G6PDx (cat#MC205271, OriGene Technologies, Rockville, MD, USA), or untagged mouse GFPT1 (cat#MC201036, OriGene Technologies, Rockville, MD, USA) vectors followed by standard in vivo muscle gene transfer/ electroporation p
FigureFigureFigure
WB
Showing 1-1 of 1 papers.
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