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Nxf1 (NM_016813) Mouse Untagged Clone

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SKU
MC200759
Nxf1 (untagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $576.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nxf1
Synonyms Mex67; Mvb1; Tap
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC005594 sequence for NM_016813
CGACGTGCTTCGCCGGATAAATGCGGTGGCGCCGGTCGTAGGAGAGATTCTGTCCTGAGCGCTAAGGCTC CTTGGCGTTTGGTGGCGTCAGGTTCTGCGCCTGAGCCCGCCCCTGTACCTGCCTGTGCCATGGCGGACGA AGGGAAGTCATACAACGAGCATGATGACCGTGTTAGTTTCCCTCAAAGAAGAAAGAAAGGCCGGGGGCCT TTCCGATGGAAGTGTGGTGAGGGGAATCGTAGGTCTGGAAGAGGTGGTTCTGGTGTACAGTCTTCCCGAT TTGAGGAAGATGATGGCGATGTAGCAATGAATGATCCCCAGGATGGCCCCCGAGTAAGATACAATCCCTA TACCAACCGGCCTAATCGTCGGGGAGATGGTTGGCATGATCGAGATCGAATTCATATTACTGTACGGAGA GACAGAGCTCCTGCAGAGAGAGGAGGGGCTGGCACCAGTCAAGATGGGACCACCAAGAACTGGTTCAAGA TTACAATTCCTTATGGTAGAAAGTATGACAAAACGTGGCTTCTGAGCATGATTCAGAGCAAATGCAGTGT CCCCTTCAATCCCATCGAGTTTCACTATGAAAATACACGGGCCCATTTTTTTGTGGAGGATGCCACTACT GCTTCTGCATTAAAGGGTGTCAACCATAAGATCCAGGATCGAGAAAACAGAAGGATATCTATCATCATCA ATGCTTCTGCTCCACCCTACACTGTACAGAATGAACTGAAGCCCGAACAAATAGAGCAGCTCAAGCTGAT CATGAGCAAACGATATGATGGCAACCAACAAGCACTTGACCTCAAGGGGCTCCGCTCAGACCCAGATTTG GTGGCCCAGAACATCGATGTTGTTCTAAACCGCAGAAGCTGTATGGCAGCCACCCTGCGGATCATTGAAG AGAACATTCCTGAGCTATTGTCTTTGAACTTGAGCAGCAACAGGCTATACAAGCTGGATGATATGTCTAG CATTGTGCAGAAGGCGCCAAACCTAAAGACCCTAAATCTCTCCGGAAACGAATTGAAGACTGAGCGGGAA TTAGACAAGATAAAAGGGCTGAAGCTGGAAGAGCTGTGGCTTGACAGAAACCCCATGTGTGACAACTTCG GAGACCAGTCCAGCTATATCAGCGCCATTCGCGAACGATTTCCCAAGTTATTACGCCTGGATGGCCATGA GCTACCCCCTCCAATTTCCTTTGATGTCGAAGCCCCTACAATGTTACCACCCTGCAAGGGAAGCTATTTT GGAACCGAGAACCTAAAGAGTCTGGTCCTGCGCTTCTTGCAGCAGTACTATGTAATTTATGACTCAGGAG ACCGCCAGGGTCTCCTGTATGCCTACCATGATGGCGCCTGTTGCTCGCTAAGCATTCCTTACAACCCTCA GAATCCTGTCCGAAAAAACTTAGCTGAATATGTCAAAGACAGCAGAAATGTGAAGAAGCTTAAAGAACCC ACCCAGCGGTTCCGACTCTTAAAGCATACACGTCTTAATGTCGTTGCCTTCCTCAATGAGTTACCCAAAA CTCAACATGACGTTAACGCCTTCGTGGTAGATATAAGTGCCCAAACAAGCACATTACTGTGTTTTTCTGT CAATGGAGTCTTCAAGGAAGTGGATGGGAAGTCTCGAGATTCTTTACGTGCTTTCACAAGAACGTTCATT GCTGTTCCTGCCAGCAATTCAGGGCTATGTATTGTAAATGATGAATTATTTGTGCGGAATGCCAGCCCTG AAGAAATCCAAAGAGCCTTCGCCATGTCTGCACCCACACCTTCCTCCAGTCCAGTACCCACGCTTTCCCC AGAACAGCAGGAAATGTTGCAAGCATTTTCTACCCAGTCTGGCATGAACCTTGAGTGGTCTCAGAAGTGC CTTCAAGACAATAACTGGGACTACACCAGATCTGCTCAGGCCTTCACTCTTCTCAAGGCCAAGGGAGAGA TTCCAGAAGTAGCGTTTATGAAATGATCACAAGAAAAGTCCCCTTGTAAATAGCCCCTGGATAATATTGT CTGGCTGTCATCTGCCGTCTCCTGTCCAGCCCGAGGCCACCCTGTAACTGTGACTGAGGAGGAAGGGCTG CTTCATCCTCCTCACCTACCTTCTGGAAGACTTAGAGAAGACTTCCGGAAGATTGAGCCTCATGGTGCCA GGAAGCCAAAGCTTACTTTGTAGAGCTGACACTAAACTACCCGAAGGACTTAGGTGCTCTGTGTACTTAA CCCCAGGATTCCCTTTACTTTTTAAGATAAAGAGTGATATTGTAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Restriction Sites RsrII-NotI |
ACCN NM_016813
Insert Size 1857 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005594, AAH05594
RefSeq Size 2312 bp
RefSeq ORF 1857 bp
Locus ID 53319
UniProt ID Q99JX7
Cytogenetics 19 5.5 cM
Summary Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Documents
Resources
"NM_016813" in other vectors (4)
SKU Description Size Price
MG209464 Nxf1 (tGFP-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1) 10 ug
$776.00
MR209464 Nxf1 (Myc-DDK-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1) 10 ug
$289.00 $576.00
MR209464L3 Lenti ORF clone of Nxf1 (Myc-DDK-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1) 10 ug
$876.00
MR209464L4 Lenti ORF clone of Nxf1 (mGFP-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1) 10 ug
$876.00

file-alt Citations

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