Ythdf2 (NM_145393) Mouse Untagged Clone
$540.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Ythdf2 |
| Synonyms | 9430020E02Rik; HGRG8; NY-REN-2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | RsrII-NotI | |
| ACCN | NM_145393 |
| Insert Size | 1740 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC014797, AAH14797 |
| RefSeq Size | 2079 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 213541 |
| UniProt ID | Q91YT7 |
| Cytogenetics | 4 D2.3 |
| Summary | Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability (PubMed:28867294, PubMed:29855337). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed:28867294, PubMed:29855337). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context (PubMed:30065315, PubMed:29855337). M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex (By similarity). Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs (By similarity). Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development (PubMed:28867294). Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation (PubMed:30065315, PubMed:30150673). Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets (PubMed:29855337). Regulates circadian regulation of hepatic lipid metabolism: acts by promoting m6A-dependent degradation of PPARA transcripts (By similarity). Regulates the innate immune response to infection by inhibiting the type I interferon response: acts by binding to m6A-containing IFNB transcripts and promoting their degradation (PubMed:30559377). Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
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Resources
"NM_145393" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG209085 | Ythdf2 (tGFP-tagged) - Mouse YTH domain family 2 (Ythdf2) | 10 ug |
$489.00
$740.00
|
|
| MR209085 | Ythdf2 (Myc-DDK-tagged) - Mouse YTH domain family 2 (Ythdf2) | 10 ug |
$289.00
$540.00
|
|
| MR209085L3 | Lenti ORF clone of Ythdf2 (Myc-DDK-tagged) - Mouse YTH domain family 2 (Ythdf2) | 10 ug |
$840.00
|
|
| MR209085L4 | Lenti ORF clone of Ythdf2 (mGFP-tagged) - Mouse YTH domain family 2 (Ythdf2) | 10 ug |
$840.00
|
|
Citations
Suppression of m6A reader Ythdf2 promotes hematopoietic stem cell expansion
Li, Qian, Shao et al
Cell Res. (2018) 28 (9), 904-917 DOI: 10.1038/s41422-018-0072-0
Li, Qian, Shao et al
Cell Res. (2018) 28 (9), 904-917 DOI: 10.1038/s41422-018-0072-0
Showing 1-1 of 1 papers.
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