Cnot7 (NM_011135) Mouse Untagged Clone

SKU MC200486

Cnot7 (untagged) - Mouse CCR4-NOT transcription complex, subunit 7 (Cnot7), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Cnot7
• Synonyms: AU022737; CAF-1; Caf1; Pop2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006021 sequence for NM_011135; GGCCGAGCGCCGCTCCAGCTCCCCTTTCCCCCTTTCGCAGGGCGAGAGGCGTCTATGGGGCTCCCGCCGT CGCCGCCGGTACCGCCACCGCCACCGCCGCCGCCGGGTGCTAGCTCTATGGCGAGGAGGAGGAGGAGGAG CGCGAGCTCAGAGACCCAAGTGCATAAGTAAAGGGTAAAGTATTTTTATGACACAAATCTTCAATCAAGT ATAACATTTTGATGCTCCACATCTAGACTCCTTGTGCTGTCACTATGCCAGCAGCAACCGTAGATCATAG CCAAAGAATTTGTGAAGTTTGGGCTTGTAACCTGGATGAAGAGATGAAGAAAATCCGTCAAGTTATCCGA AAATATAATTATGTTGCTATGGACACCGAGTTTCCAGGCGTTGTTGCAAGACCCATTGGAGAATTCAGAA GCAATGCTGACTATCAGTACCAACTGTTGCGGTGTAATGTAGACTTGTTAAAGATAATCCAGCTCGGACT GACCTTTATGAATGAACAGGGAGAATACCCTCCAGGAACGTCAACTTGGCAGTTTAACTTTAAGTTTAAT TTGACGGAGGACATGTATGCTCAGGACTCTATAGAGCTACTAACAACATCTGGTATCCAGTTTAAAAAAC ACGAGGAGGAAGGAATTGAGACCCAATATTTTGCAGAACTTCTTATGACTTCAGGAGTGGTTCTTTGTGA AGGGGTCAAATGGCTATCATTTCACAGTGGTTATGACTTTGGCTATTTAATCAAAATTCTGACCAACTCT AACTTGCCTGAGGAAGAACTTGATTTCTTTGAGATCCTTCGGTTATTTTTTCCTGTCATTTATGATGTGA AGTACCTCATGAAGAGCTGCAAAAATCTCAAAGGTGGATTACAGGAAGTTGCTGAGCAGTTAGAGCTGGA GCGCATAGGCCCTCAGCACCAGGCAGGATCTGACTCACTGCTTACAGGAATGGCCTTTTTCAAAATGAGA GAAATGTTCTTTGAAGATCACATTGATGATGCCAAATACTGTGGTCACTTATATGGCCTTGGTTCTGGCT CATCCTATGTACAGAACGGCACAGGGAATGCATATGAAGAGGAAGCCAGCAAGCAGTCATGACAGGAAAT AGTCTTCTTATTTTTATTTTATACGAGTTACACACATGCTTGTATATAGGTTTTATCATCCCTTAAATAA TAGGCAATACCCCCCCCTTTTGTAGACCTTCTCTGCTTTCAGTAATAAGTATGGCCTTTCTTAGCTATGA TCTTAATAAATTAAAGTACATTCAGGTTAAATCTGGCCTTAATTTAATACACTTCTTATCAAGCTTGTGT GACAAAGCTACATTACTGAATAATTTGATAAACTTTGCCTACTTGAGCTTGGTTTTGTTTTTCCCTTTTC CTAAATTAACTAGCATTGAGTGTAATTTATTTCCCTCTTCCATGCCTCTCCTTCTATTCTGCAGTACTTA TAGGTATTTAAGATTGTGGACCATCAAGTTTGCACTTTAGAGAGTGATTTCAATCCTCTATTTTATCAGA AATTGTGATTTTTCTTGCTCTTAGCTAGAAAAATAACCATCTACCACCTTTTTACAGTATTTGCTTGTTT TTGACATTCAGAGTACAGCACGTGCATTGTGCAAATTGTTCAACAGGAATAACAGAAAAAGACAAGGAAC TACTGAAGAGCCTAGTAACTACTGTTTCTGTGTGTAGTGTTTAGCCTTCCAAGCACATCTCATGTCTGTT GGTTTCTAATTGGCATGCATAGGCTGCTATGTGACTGAAGAATTTCAAACCAGCTTTACACCCTTCAGGA AGAAGCCCTGTGATTGGCTATCTCCTCTCTGCCAGAAGGCTGGCCATTAAGATGTTGGAGCTCCAGTTTT ATGACAGCACCGCCTCCCTTGATCGGCTTCCTTTTACTCCATCACTTTTACCCTTACTGTTTTATTTTAT AAACATATTTATGATGTTCTTGATTTGTTAACTACACAAATCAATTTCATTAAAGTCCAAAGATAACAAG TCTTAAGGTACATTTTATATCAATAAAAATACAAAATAAATGTGATTTCATAAATGCTACAAAAATAAAT AATGGAGAGATTTGGTGCAAAACAACAAAATGCAAAAAATATATATAAATACACACATATATAGCTAATA AAATTACCTGAGGAGTGTAATGCTTGTTTATTTTTTTGTGTATATCTTTGCAATCTATTTTATATATATT GACAAGAGACTGTGAAATATTTAGCCATGCAGAATATGTGACCAGACCAGAGCATGTGTTGGAAGATTTT GGTGAACATTAACTCTACCCTGAAGTGATGGACTACAAGTTGTAATGTGTGTTATCTACACTTCAATCGA TATTAGCAAGTCTCCAACTGTTAGCAACATAGTTCGTTTCCCTTGTACATTCTGTAAACATGATATGAAA ATGCTGTACCTGGGCGGTCCTTTTTAAACTAAACATTATTTGCAAAACAGAGGGTATTATTTGTTTTTAA AGCTTTTGTAAATAAAGGCTTCAAAAATGTTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011135
• Insert Size: 858 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC006021, AAH06021
• RefSeq Size: 2679 bp
• RefSeq ORF: 858 bp
• Locus ID: 18983
• UniProt ID: Q60809
• Cytogenetics: 8 A4
• Summary: Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes isoform . Variants 1, 2 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.