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Nhej1 (NM_029342) Mouse Untagged Clone

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SKU
MC200480
Nhej1 (untagged) - Mouse nonhomologous end-joining factor 1 (Nhej1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nhej1
Synonyms 1700029B21Rik; cernunnos; XLF
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC006063 sequence for NM_029342
CTCTCAGTTTGCGCACCAGGACAGGCCCGCCTTGAGGAGGGTGGGAGTTCCCGCAGCCGTTGACGTTGAC ATGGAGGTGTGAGCCGCCCTCTTGAAGGCTGACTTTCCTGAGGACACTGTGCTGAATTAGGACTTTTCTG CAGATGGAAGAGCTAGAGCAAGACCTGTTGCTGCAGCCATGGGCATGGTTACAACTTGCGGAGAACTCAC TCTTAGCCAAGGTGTCTATCACCAAGCACGGTTATGCCTTGCTGATTTCGGATCTTCAACAGGTGTGGCA TGAACAGGTGGACACTTCGGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGCCTCACTGCGCCTCCT GCAGCTTTGCTCTGTCACCTGGATGAAGCACTTCGCCCACTGTTTAAAGATTCTGCTCACCCTAGCAAAG CTACTTTCTCCTGTGACCGAGGAGAGGAGGGACTGATCCTGCGGGTGCAGAGTGAGCTCTCGGGTCTTCC CTTCAGTTGGCATTTCCACTGTATTCCAGCTAGTTCTTCACTGGTCTCTCAGCATTTGATTCATCCTCTG ATGGGTGTGAGCCTGGCACTGCAGAGTCATGTGAGGGAGCTAGCAGCATTGCTTCGGATGAAGGACCTTG AGATCCAGGCCTACCAGGAGAGTGGGGCTGTGCTGAGCCGAAGTCGATTGAAGACAGAGCCATTTGAAGA AAATTCTTTCTTGGAACAGTTTATGGCAGAGAAATTGCCAGAGGCGTGTGCTGTTGGTGATGGAAAGCCA TTTGCCATGAGTCTGCAGAGTCTGTATGTGGCAGTTACAAAACAGCAGATCCAAGCAAGGCAGGCACATA AAGACTCTGGAGAGACTCAGGCATCAAGCAGCACCTCCCCTCGAGGAACTGATAACCAGCCAGAAGAGCC GGTCTCCCTCCCTTCCACCCTCTCAGAACCTGAATATGAGCCTGTGGCTGCTTCAGGCCCTATGCATAGA GCTCAGCTGGTGAAGTCCAAGAGGAAGAAGCCCAGGGGACTCTTCAGTTAACTCTCCATGTCTCAGCCAC CACGAAGAGAGCAAAACCACTTCCTAGCATCACCTGGAAGGAGCTTGCATGGCAGAGCATCCCTCAGAGG CCTGAGTTTGTGTTGACAGGCCACCTGGATGGGGACTTTACCAGAGGAAGCCTGACTCCAACTCACTGGC ATTCCGGATATGAGTGACACCAGCTCCTTGCTTGGGTAGTGTTCTTTCTTTAGAACTAGCACCCTATGGC AAACTTAGGTACATGAAGCAAGTTTACAAAGAGGCGCATGCCCAGAACTCCTGGCTGCCTTTCACAGGGG TCTCTCTCTCTCAACACAGAATAAAGGATGCTCACACTCCTCGACTGTTGAGGTTACCTGTGATCTCTGG ATCCCAGAATCCCCCTAGAACAGAACTGGGTGTCCCCTAGTCCTGTTGGGTCACATCTGAGCTATCAGCT CCATCCTACAGACTCTTCGTCAGCTTCTCTCATGAGGCTGAGGTCAGCCACAGTTAGAAGCCTTGATCTT GAGAACTCTCTCCATGTTGGTACAAGACAGATTACATTTCTGTCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_029342
Insert Size 888 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC006063, AAH06063
RefSeq Size 1634 bp
RefSeq ORF 888 bp
Locus ID 75570
UniProt ID Q3KNJ2
Cytogenetics 1 C4
Summary DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary (PubMed:17360556). Binds DNA in a length-dependent manner (By similarity).UniProtKB/Swiss-Prot Function
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"NM_029342" in other vectors (4)
SKU Description Size Price
MG204061 Nhej1 (tGFP-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) 10 ug
$500.00
MR204061 Nhej1 (Myc-DDK-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) 10 ug
$289.00 $300.00
MR204061L3 Lenti ORF clone of Nhej1 (Myc-DDK-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) 10 ug
$600.00
MR204061L4 Lenti ORF clone of Nhej1 (mGFP-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) 10 ug
$600.00

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