Cloning Accessories

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  1. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme joins blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA and some DNA/RNA hybrids.
  2. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme joins blunt-end and cohesive-end termini as well as repairs single-stranded nicks in duplex DNA and some DNA/RNA hybrids.
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