Human Transcription Factors Clone Set
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TCTF101
Human transcription factors clone set I, containing 90 cDNA clones in mammalian expression vector
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$1,201.00
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Bisphenol A derivatives act as novel coactivator binding inhibitors for estrogen receptor β
Iwamoto, Masuya, Hosose et al
J Biol Chem (2021) 297 (5), 101173 DOI: 10.1016/j.jbc.2021.101173
Iwamoto, Masuya, Hosose et al
J Biol Chem (2021) 297 (5), 101173 DOI: 10.1016/j.jbc.2021.101173
Cited by 30
AI Summary: OriGene Technologies contributed to this study by providing the human ERβ cDNA, which was used to express and purify the ERβ ligand-binding domain for receptor-binding assays.
Snippet: rchased from PerkinElmer. ERβ expression and purification The LBD of ERβ (amino acids 263–530) was expressed as a GST-fused protein for receptor-binding assays. Human ERβ cDNA was obtained from OriGene Technologies. The cDNA of ERβ-LBD was amplified using PCR and subcloned into a pGEX-6p-1 expression vector. The expression of GST-fused ERβ-LBD was induced by 1 mM IPTG in Escherichia coli;Human ER...
A signal transduction approach for multiplexed detection of transcription factors by integrating DNA nanotechnology, multi-channeled isothermal amplification, and chromatography
Chen, Yan, Yang et al
J Chromatogr A (2020) 1624, 461148 DOI: 10.1016/j.chroma.2020.461148
Chen, Yan, Yang et al
J Chromatogr A (2020) 1624, 461148 DOI: 10.1016/j.chroma.2020.461148
Cited by 14
AI Summary: In this paper, OriGene products—including recombinant human p50 (TP303565), p53 (TP315322), AP-1 (TP305331), MITF (TP317566), and c-Myc (TP320236) proteins—were used as target transcription factors to validate and quantify the multiplexed detection capability of the described PAC system.
Magnetic Synthetic Receptors for Selective Clean-Up in Protein Biomarker Quantification
McKitterick, Braathen, Switnicka-Plak et al
J. Proteome Res. (2020)
McKitterick, Braathen, Switnicka-Plak et al
J. Proteome Res. (2020)
Cited by 14
AI Summary: OriGene Technologies provided the human cDNA used to clone ProGRP isoform 1, which was essential for producing the target protein in this study.
Snippet: 122 Norway. ProGRP isoform 1 was cloned from human cDNA (Origene technologies) and 123 expressed in Escherichia coli (Promega) via pGEX-6P-3 constructs (GE Healthcare) and Page 5 of 34 ACS Paragon Plus Environment Journal of Proteome Research
AIFM2 blocks ferroptosis independent of ubiquinol metabolism
Dai, Zhang, Cong et al
Biochem. Biophys. Res. Commun. (2020) 523 (4), 966-971 DOI: 10.1016/j.bbrc.2020.01.066
Dai, Zhang, Cong et al
Biochem. Biophys. Res. Commun. (2020) 523 (4), 966-971 DOI: 10.1016/j.bbrc.2020.01.066
Cited by 232
AI Summary: OriGene’s Human AIFM2-cDNA was used in this study to enable gene transfection experiments investigating the role of AIFM2 in blocking ferroptosis in cancer cells.
Snippet: Aldrich. Human AIFM2-cDNA was purchased from OriGene. RNAi and gene transfection were performed using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer's instructions. 2.4. Western blot. Western;- Aldrich. Human AIFM2-cDNA was purchased from OriGene. RNAi and gene transfection were performed using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufactu...
E2F1 acetylation directs p300/CBP-mediated histone acetylation at DNA double-strand breaks to facilitate repair
Manickavinayaham, Vélez-Cruz, Biswas et al
Nat Commun (2019) 10 (1), 4951 DOI: 10.1038/s41467-019-12861-8
Manickavinayaham, Vélez-Cruz, Biswas et al
Nat Commun (2019) 10 (1), 4951 DOI: 10.1038/s41467-019-12861-8
AI Summary: OriGene's human cDNA library was used as the source for cloning cDNAs encoding protein domains studied in this paper, enabling the investigation of E2F1 acetylation and its role in recruiting p300/CBP to DNA double-strand breaks for efficient repair.
Snippet: accession numbers, and regions cloned can be found elsewhere68,69. The complementary DNAs (cDNAs) encoding the domains listed were cloned into the pGEX-6P1 vectors by PCR using a human cDNA library (Origene, MD) and verified by DNA sequencing68. Peptides were synthesized by W. M. Keck Biotechnology Resource Center (New Haven, CT) and CPC Scientific (Sunnyvale, CA). E2F1 peptides corresponding to
Immunocapture sample clean-up in determination of low abundant protein biomarkers - a feasibility study of peptide capture by anti-protein antibodies
Levernæs, Farhat, Oulie et al
RSC Adv (2019) 9 (60), 34902-34911 DOI: 10.1039/c9ra05071j
Levernæs, Farhat, Oulie et al
RSC Adv (2019) 9 (60), 34902-34911 DOI: 10.1039/c9ra05071j
Cited by 17
AI Summary: OriGene contributed monoclonal antibodies E146, E149, and M18 to this study, which were evaluated for their effectiveness in capturing proteotypic epitope peptides of ProGRP during immunocapture sample clean-up for mass spectrometry-based biomarker analysis.
Showing 1-6 of 62 papers.
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