Human Secreted Clone Set

Snippet: N-α4 radioligand binding assay Serum IL-22 and IFN-α4 autoantibodies were measured by immunoprecipitation using radiolabeled IL-22 and IFN-α4 proteins. Human cDNA (IL-22 RC209995, IFN-a4 SC304903, OriGene) was cloned into pTNT vector (L5610, Promega). The vectors were used for in vitro transcription and translation with a Sp6 TNT coupled reticulocyte-lysate system (Promega) in the presence of 3

Snippet: 2.9. CD73 Transfection. CD73 was transfected into CD73neg BrCa cell lines using a human cDNA ORF clone purchased from Origene, Rockville. Transfection was performed according to the manufactures' instructions (Invitrogen, Waltham, MA, USA) Cached

Snippet: Human XIST clone (NR_001564.2, bp 61-5349) was purchased from Origene (SC312039) THP-1 and THP1-XBlue cells were transfected with human XIST cDNA clone (5275 bp, Origene) using Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol;- Human XIST clone (NR_001564.2, bp 61-5349) was purchased from Origene (SC312039) THP-1 and THP1-XBlue cells were transfected with human XIST cDNA clone (5275 bp, Origene) using Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol

Snippet: Cells were maintained at 37 °C in a humidified atmosphere of 95% air/5% CO 2 . pcDNA3.1-v5-hYSK1 was generated by PCR using the human cDNA clone-hYSK1 (Origene Technologies, Rockville, MD, USA) as a template Cached

Snippet: The AdLTR 2 EF1α-hNRTN vector used for these experiments, here abbreviated to AdNRTN for simplicity, is a hybrid E1-deleted serotype 5 recombinant adenoviral vector with a human NRTN cDNA (hNRTN;Origene Technologies, Rockville, MD, USA), which can persist

Snippet: U6 snRNA was used for normalization (Assay ID 001973, Applied Biosystems). Luciferase reporter assay. Human XIST cDNA clone (Origene, Rockville, MD, USA) was subcloned using the Sac I and Xho I restriction sites into the pmirGLO vector (Promega, Madison