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MET (NM_001127500) Human 3' UTR Clone

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SKU
SC218300
3' UTR clone of met proto-oncogene (hepatocyte growth factor receptor) (MET) transcript variant 1 for miRNA target validation
  $813.00
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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol MET
Synonyms AUTS9; c-Met; DFNB97; HGFR; RCCP2
ACCN NM_001127500
Insert Size 2306 bp
Sequence Data
Insert Sequence
>SC218300 3' UTR clone of NM_001127500
The sequence shown below is from the reference sequence of NM_001127500. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

TGATGAGGTGGACACACGACCAGCCTCCTTCTGGGAGACATCATAGTGCTAGTACTATGTCAAAGCAACA
GTCCACACTTTGTCCAATGGTTTTTTCACTGCCTGACCTTTAAAAGGCCATCGATATTCTTTGCTCTTGC
CAAAATTGCACTATTATAGGACTTGTATTGTTATTTAAATTACTGGATTCTAAGGAATTTCTTATCTGAC
AGAGCATCAGAACCAGAGGCTTGGTCCCACAGGCCACGGACCAATGGCCTGCAGCCGTGACAACACTCCT
GTCATATTGGAGTCCAAAACTTGAATTCTGGGTTGAATTTTTTAAAAATCAGGTACCACTTGATTTCATA
TGGGAAATTGAAGCAGGAAATATTGAGGGCTTCTTGATCACAGAAAACTCAGAAGAGATAGTAATGCTCA
GGACAGGAGCGGCAGCCCCAGAACAGGCCACTCATTTAGAATTCTAGTGTTTCAAAACACTTTTGTGTGT
TGTATGGTCAATAACATTTTTCATTACTGATGGTGTCATTCACCCATTAGGTAAACATTCCCTTTTAAAT
GTTTGTTTGTTTTTTGAGACAGGATCTCACTCTGTTGCCAGGGCTGTAGTGCAGTGGTGTGATCATAGCT
CACTGCAACCTCCACCTCCCAGGCTCAAGCCTCCCGAATAGCTGGGACTACAGGCGCACACCACCATCCC
CGGCTAATTTTTGTATTTTTTGTAGAGACGGGGTTTTGCCATGTTGCCAAGGCTGGTTTCAAACTCCTGG
ACTCAAGAAATCCACCCACCTCAGCCTCCCAAAGTGCTAGGATTACAGGCATGAGCCACTGCGCCCAGCC
CTTATAAATTTTTGTATAGACATTCCTTTGGTTGGAAGAATATTTATAGGCAATACAGTCAAAGTTTCAA
AATAGCATCACACAAAACATGTTTATAAATGAACAGGATGTAATGTACATAGATGACATTAAGAAAATTT
GTATGAAATAATTTAGTCATCATGAAATATTTAGTTGTCATATAAAAACCCACTGTTTGAGAATGATGCT
ACTCTGATCTAATGAATGTGAACATGTAGATGTTTTGTGTGTATTTTTTTAAATGAAAACTCAAAATAAG
ACAAGTAATTTGTTGATAAATATTTTTAAAGATAACTCAGCATGTTTGTAAAGCAGGATACATTTTACTA
AAAGGTTCATTGGTTCCAATCACAGCTCATAGGTAGAGCAAAGAAAGGGTGGATGGATTGAAAAGATTAG
CCTCTGTCTCGGTGGCAGGTTCCCACCTCGCAAGCAATTGGAAACAAAACTTTTGGGGAGTTTTATTTTG
CATTAGGGTGTGTTTTATGTTAAGCAAAACATACTTTAGAAACAAATGAAAAAGGCAATTGAAAATCCCA
GCTATTTCACCTAGATGGAATAGCCACCCTGAGCAGAACTTTGTGATGCTTCATTCTGTGGAATTTTGTG
CTTGCTACTGTATAGTGCATGTGGTGTAGGTTACTCTAACTGGTTTTGTCGACGTAAACATTTAAAGTGT
TATATTTTTTATAAAAATGTTTATTTTTAATGATATGAGAAAAATTTTGTTAGGCCACAAAAACACTGCA
CTGTGAACATTTTAGAAAAGGTATGTCAGACTGGGATTAATGACAGCATGATTTTCAATGACTGTAAATT
GCGATAAGGAAATGTACTGATTGCCAATACACCCCACCCTCATTACATCATCAGGACTTGAAGCCAAGGG
TTAACCCAGCAAGCTACAAAGAGGGTGTGTCACACTGAAACTCAATAGTTGAGTTTGGCTGTTGTTGCAG
GAAAATGATTATAACTAAAAGCTCTCTGATAGTGCAGAGACTTACCAGAAGACACAAGGAATTGTACTGA
AGAGCTATTACAATCCAAATATTGCCGTTTCATAAATGTAATAAGTAATACTAATTCACAGAGTATTGTA
AATGGTGGATGACAAAAGAAAATCTGCTCTGTGGAAAGAAAGAACTGTCTCTACCAGGGTCAAGAGCATG
AACGCATCAATAGAAAGAACTCGGGGAAACATCCCATCAACAGGACTACACACTTGTATATACATTCTTG
AGAACACTGCAATGTGAAAATCACGTTTGCTATTTATAAACTTGTCCTTAGATTAATGTGTCTGGACAGA
TTGTGGGAGTAAGTGATTCTTCTAAGAATTAGATACTTGTCACTGCCTATACCTGCAGCTGAACTGAATG
GTACTTCGTATGTTAATAGTTGTTCTGATAAATCATGCAATTAAAGTAAAGTGATGCAACATCTTG

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001127500.1
Locus ID 4233
Summary This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. provided by RefSeq, May 2016
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Documents
Resources

file-alt Citations

An integrated genomic analysis of papillary renal cell carcinoma type 1 uncovers the role of focal adhesion and extracellular matrix pathways
Wala, Karamchandani, Saleeb et al
Mol Oncol (2015) 9 (8), 1667-77 DOI: 10.1016/j.molonc.2015.04.007
Cited by 23
AI Summary: In this study, OriGene products such as the miR-199a-3p mimic and MET luciferase reporter plasmid were used to experimentally validate the role of miR-199a-3p and its regulation of key genes in the focal adhesion and extracellular matrix pathways in papillary renal cell carcinoma type 1.
Showing 1-1 of 1 papers.
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