TNFAIP3 (NM_006290) Human 3' UTR Clone

SKU SC217355

3' UTR clone of tumor necrosis factor alpha-induced protein 3 (TNFAIP3) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: TNFAIP3
• Synonyms: A20; AISBL; OTUD7C; TNFA1P2
• ACCN: NM_006290
• Insert Size: 2014 bp
• Sequence Data:
  Insert Sequence

  >SC217355 3' UTR clone of NM_006290; The sequence shown below is from the reference sequence of NM_006290. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon ; ; ; CAATTGGCAGAGCTCAGAATTCAAGCGATCGC; ; ACGGCTACTGCAACGAATGCTTTCAGTTCAAGCAGATGTATGGCTAACCGGAAACAGGTGGGTCACCTCC; TGCAAGAAGTGGGGCCTCGAGCTGTCAGTCATCATGGTGCTATCCTCTGAACCCCTCAGCTGCCACTGCA; ACAGTGGGCTTAAGGGTGTCTGAGCAGGAGAGGAAAGATAAGCTCTTCGTGGTGCCCACGATGCTCAGGT; TTGGTAACCCGGGAGTGTTCCCAGGTGGCCTTAGAAAGCAAAGCTTGTAACTGGCAAGGGATGATGTCAG; ATTCAGCCCAAGGTTCCTCCTCTCCTACCAAGCAGGAGGCCAGGAACTTCTTTGGACTTGGAAGGTGTGC; GGGGACTGGCCGAGGCCCCTGCACCCTGCGCATCAGGACTGCTTCATCGTCTTGGCTGAGAAAGGGAAAA; GACACACAAGTCGCGTGGGTTGGAGAAGCCAGAGCCATTCCACCTCCCCTCCCCCAGCATCTCTCAGAGA; TGTGAAGCCAGATCCTCATGGCAGCGAGGCCCTCTGCAAGAAGCTCAAGGAAGCTCAGGGAAAATGGACG; TATTCAGAGAGTGTTTGTAGTTCATGGTTTTTCCCTACCTGCCCGGTTCCTTTCCTGAGGACCCGGCAGA; AATGCAGAACCATCCATGGACTGTGATTCTGAGGCTGCTGAGACTGAACATGTTCACATTGACAGAAAAA; CAAGCTGCTCTTTATAATATGCACCTTTTAAAAAATTAGAATATTTTACTGGGAAGACGTGTAACTCTTT; GGGTTATTACTGTCTTTACTTCTAAAGAAGTTAGCTTGAACTGAGGAGTAAAAGTGTGTACATATATAAT; ATACCCTTACATTATGTATGAGGGATTTTTTTAAATTATATTGAAATGCTGCCCTAGAAGTACAATAGGA; AGGCTAAATAATAATAACCTGTTTTCTGGTTGTTGTTGGGGCATGAGCTTGTGTATACACTGCTTGCATA; AACTCAACCAGCTGCCTTTTTAAAGGGAGCTCTAGTCCTTTTTGTGTAATTCACTTTATTTATTTTATTA; CAAACTTCAAGATTATTTAAGTGAAGATATTTCTTCAGCTCTGGGGAAAATGCCACAGTGTTCTCCTGAG; AGAACATCCTTGCTTTGAGTCAGGCTGTGGGCAAGTTCCTGACCACAGGGAGTAAATTGGCCTCTTTGAT; ACACTTTTGCTTGCCTCCCCAGGAAAGAAGGAATTGCATCCAAGGTATACATACATATTCATCGATGTTT; CGTGCTTCTCCTTATGAAACTCCAGCTATGTAATAAAAAACTATACTCTGTGTTCTGTTAATGCCTCTGA; GTGTCCTACCTCCTTGGAGATGAGATAGGGAAGGAGCAGGGATGAGACTGGCAATGGTCACAGGGAAAGA; TGTGGCCTTTTGTGATGGTTTTATTTTCTGTTAACACTGTGTCCTGGGGGGGCTGGGAAGTCCCCTGCAT; CCCATGGTACCCTGGTATTGGGACAGCAAAAGCCAGTAACCATGAGTATGAGGAAATCTCTTTCTGTTGC; TGGCTTACAGTTTCTCTGTGTGCTTTGTGGTTGCTGTCATATTTGCTCTAGAAGAAAAAAAAAAAAGGAG; GGGAAATGCATTTTCCCCAGAGATAAAGGCTGCCATTTTGGGGGTCTGTACTTATGGCCTGAAAATATTT; GTGATCCATAACTCTACACAGCCTTTACTCATACTATTAGGCACACTTTCCCCTTAGAGCCCCCTAAGTT; TTTCCCAGACGAATCTTTATAATTTCTTTCCAAAGATACCAAATAAACTTCAGTGTTTTCATCTAATTCT; CTTAAAGTTGATATCTTAATATTTTGTGTTGATCATTATTTCCATTCTTAATGTGAAAAAAAGTAATTAT; TTATACTTATTATAAAAAGTATTTGAAATTTGCACATTTAATTGTCCCTAATAGAAAGCCACCTATTCTT; TGTTGGATTTCTTCAAGTTTTTCTAAATAAATGTAACTTTTCACAAGAGTCAAC; ; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006290.2
• Locus ID: 7128
• Summary: This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]