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UBE3A (NM_000462) Human 3' UTR Clone

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SKU
SC216901
3' UTR clone of ubiquitin protein ligase E3A (UBE3A) transcript variant 2 for miRNA target validation
  $813.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol UBE3A
Synonyms ANCR; AS; E6-AP; EPVE6AP; HPVE6A
ACCN NM_000462
Insert Size 1933 bp
Sequence Data
Insert Sequence
>SC216901 3’UTR clone of NM_000462
The sequence shown below is from the reference sequence of NM_000462. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
ACGTATGCCAAAGGATTTGGCATGCTGTAAAACAAAACAAAACAAAATAAAACAAAAAAAAGGAAGGAA
AAAAAAAGAAAAAATTTAAAAAATTTTAAAAATATAACGAGGGATAAATTTTTGGTGGTGATAGTGTCC
CAGTACAAAAAGGCTGTAAGATAGTCAACCACAGTAGTCACCTATGTCTGTGCCTCCCTTCTTTATTGG
GGACATGTGGGCTGGAACAGCAGATTTCAGCTACATATATGAACAAATCCTTTATTATTATTATAATTA
TTTTTTTGCGTGAAAGTGTTACATATTCTTTCACTTGTATGTACAGAGAGGTTTTTCTGAATATTTATT
TTAAGGGTTAAATCACTTTTGCTTGTGTTTATTACTGCTTGAGGTTGAGCCTTTTGAGTATTTAAAAAA
TATATACCAACAGAACTACTCTCCCAAGGAAAATATTGCCACCATTTGTAGACCACGTAACCTTCAAGT
ATGTGCTACTTTTTTGTCCCTGTATCTAACTCAAATCAGGAACTGTATTTTTTTTAATGATTTGCTTTT
GAAACTTGAAGTCTTGAAAACAGTGTGATGCAATTACTGCTGTTCTAGCCCCCAAAGAGTTTTCTGTGC
AAAATCTTGAGAATCAATCAATAAAGAAAGATGGAAGGAAGGGAGAAATTGGAATGTTTTAACTGCAGC
CCTCAGAACTTTAGTAACAGCACAACAAATTAAAAACAAAAACAACTCATGCCACAGTATGTCGTCTTC
ATGTGTCTTGCAATGAACTGTTTCAGTAGCCAATCCTCTTTCTTAGTATATGAAAGGACAGGGATTTTT
GTTCTTGTTGTTCTCGTTGTTGTTTTAAGTTTACTGGGGAAAGTGCATTTGGCCAAATGAAATGGTAGT
CAAGCCTATTGCAACAAAGTTAGGAAGTTTGTTGTTTGTTTATTATAAACAAAAAGCATGTGAAAGTGC
ACTTAAGATAGAGTTTTTATTAATTACTTACTTATTACCTAGATTTTAAATAGACAATCCAAAGTCTCC
CCTTCGTGTTGCCATCATCTTGTTGAATCAGCCATTTTATCGAGGCACGTGATCAGTGTTGCAACATAA
TGAAAAAGATGGCTACTGTGCCTTGTGTTACTTAATCATACAGTAAGCTGACCTGGAAATGAATGAAAC
TATTACTCCTAAGAATTACATTGTATAGCCCCACAGATTAAATTTAATTAATTAATTCAAAACATGTTA
AACGTTACTTTCATGTACTATGGAAAAGTACAAGTAGGTTTACATTACTGATTTCCAGAAGTAAGTAGT
TTCCCCTTTCCTAGTCTTCTGTGTATGTGATGTTGTTAATTTCTTTTATTGCATTATAAAATAAAAGGA
TTATGTATTTTTAACTAAGGTGAGACATTGATATATCCTTTTGCTACAAGCTATAGCTAATGTGCTGAG
CTTGTGCCTTGGTGATTGATTGATTGATTGACTGATTGTTTTAACTGATTACTGTAGATCAACCTGATG
ATTTGTTTGTTTGAAATTGGCAGGAAAAATGCAGCTTTCAAATCATTGGGGGGAGAAAAAGGATGTCTT
TCAGGATTATTTTAATTAATTTTTTTCATAATTGAGACAGAACTGTTTGTTATGTACCATAATGCTAAA
TAAAACTGTGGCACTTTTCACCATAATTTAATTTAGTGGAAAAAGAAGACAATGCTTTCCATATTGTGA
TAAGGTAACATGGGGTTTTTCTGGGCCAGCCTTTAGAACACTGTTAGGGTACATACGCTACCTTGATGA
AAGGGACCTTCGTGCAACTGTAGTCATCTTAAAGGCTTCTCATCCACTGTGCTTCTTAATGTGTAATTA
AAGTGAGGAGAAATTAAATACTCTGAGGGCGTTTTATATAATAAATTCGTGAAGAAATGTGTGCTCTTCA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000462.5
Locus ID 7337
MW 74.2
Summary This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. provided by RefSeq, Jul 2008
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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