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RFK (NM_018339) Human 3' UTR Clone

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SKU
SC216871
3' UTR clone of riboflavin kinase (RFK) for miRNA target validation
  $813.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol RFK
Synonyms RIFK
ACCN NM_018339
Insert Size 1903 bp
Sequence Data
Insert Sequence
>SC216871 3’UTR clone of NM_018339
The sequence shown below is from the reference sequence of NM_018339. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
TCTAAAAGCAAAATAATGAATGGCCACTGATGAAAAATTGTATTATTTATTCATTCACTGTTTTCTAGT
GTTTCTGTGTTTATTACTGTTCAGCATCATCTTGGTTATATACTGAAAATCAAACACTTTACTACAGTT
GTGAATTAGTTTAAACCGTACAATGTTGTTATCATATCATGCTTCAACTATTAAATTAAGCCCATCATA
TCATAGCACTAAAAAGATTGAGTTAAAAATAAGATTTAAAAAATATATATTGATTAAAATGTACCACTA
GAAAGGTATTACATGTGTATAAGTATGGGTAAAAAGGCAAGTCACTAGTTTGAGATGAAAACTAAAATT
TTCATGGTAAAATGCCAGCATGTATGTACTTGTGTAGTGTGTACTTCTAAACAGAGAAGGCTTATAAAA
GTAAATGCATTAAGGCCAAGTGCAGTGGCTCACACCGGTAATCCCAGCACTTTGGGAGGCCGAGGTGGG
AGGATTGCTTGAGCTCATGAGTTCAAGACCAGCCTGGGCAACATAGCGAGACTCCATCTCTATAAATAA
AAAAAAAGGAAATGCATTAAATAGAAGGTTGATAGTTTATCATAAACCTGCGGGGAAGAAACTCAGACT
TTCTATTTTTGATATTTTGTGCATTAATACATTACAAACATGAAGATGTTTGTAAAGTTTTGTTTTAGT
CATTTTCTTTGCAGTCACATCATTGTACCACCTTCATTATTGTATAGAGCTGCTTCAATTCTGAGTCCT
CTGGACTTCCCCTGCCTATCTGTTTGTTGTTGTTGTTGAAGACAGTAAAATAAGTGTTTTCAAATCTAT
ATGTCTCCTTTTTGTTGGATAATGTTTCTTCTTTTTAATGGTAAATATTCTTGTTCTTCAACATTTTTC
TTTGGTTCTTTTTTCCTTTTTTAGGAAAAACAAAACAACAGACTTCATCCTTAGGTTTTCTCAAGATTT
AAGTGAACACATTTACACATATCAATTTCTTAAAGAACACAAAATGTTTCCTCCCTAGCAAAACTATTT
AAGAGCCAATTAAACTATGAAATAGTTATAAAATTATTTTTAATATTTAAGGCCTTGAAATTATATTCC
AAGGCAAATCTAAATGGGTGTAGTTGTATATGCACGATGTAATTTAATGCTAAAGAGTACTTACAGCTT
GAGGGAAAAGCATAAAGTTTTAGTTAAATAGCCTTCATACTGTGGATTTTTCCTCCTACTAACTAATAT
GTGGGAAATTAAGATTACAGATGCTTTGGGCCCCCTCAAACAGTACATTGCTACAGTTCACTGTAGCAA
CAGATTTGGTGGTGATGGGGGGATAGAGGTAGAAGCAGATAGAAGGTAATTTTAAAGTTAATTTATTAT
GAATCATTTTCCCCTAATCTGCATAAATGAATTAGAACTGATTTTAATAAAAATATTCTGTGTAAAAAG
TACTCAGAAAAACATCTAAAGCTGTTGGAGAAGAGGGACTCATATAAATAGTTGGATTGCGTTTTGCTT
TTTATTTCTTTATAAAAATATGTCAAAAACAGTTTTTAATTTTAGAAATCTTATACTTGAAAGAAACCT
CAATTATCAGTGGCTTTATGAAGTAAAGTTAAAAACAAACATAGGAAATGTGAAAATATTTGTGTTTGT
TTTGTCTGACTAACTTGCTGCTAAATGCTATCTTTAGGTGTGTGAAAGTTCTTTGATATGTTATTGTCC
TTTTACATCTTGTTAAATAGCCATTGCTTTTGAGAATCAGATAGTGTGTTGTTTAGGGGTTAATTGTGT
CTATATCTTTCAAAAACTTTTATATTATGTATTATAAGAAGTTACAGTAGTACCATTTTGATTAGTCTG
AATGTTTTCAGGCCTTGAAATAAATTTTGACTAAGTGATA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018339.6
Locus ID 55312
MW 73.9
Summary Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 PubMed 12623014).supplied by OMIM, Nov 2009
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