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Caspase 3 (CASP3) (NM_032991) Human 3' UTR Clone

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SKU
SC215501
3' UTR clone of caspase 3 apoptosis-related cysteine peptidase (CASP3) transcript variant beta for miRNA target validation
  $813.00
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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol Caspase 3
Synonyms CPP32; CPP32B; SCA-1
ACCN NM_032991
Insert Size 1606 bp
Sequence Data
Insert Sequence
>SC215501 3’UTR clone of NM_032991
The sequence shown below is from the reference sequence of NM_032991. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CTCACAAAAGAACTCTATTTTTATCACTAAAGAAATGGTTGGTTGGTGGTTTTTTTTAGTTTGTATGCC
AAGTGAGAAGATGGTATATTTGGTACTGTATTTCCCTCTCATTTTGACCTACTCTCATGCTGCAGAGGG
TACTTTAAGACATACTCCTTCCATCAAATAGAACCACTATGAAGCTACCTCAAACTTCCAGTCAGGTAG
TTGCAATTGAATTAAATTAGGAATAAATAAAAATGGATACTGGTGCAGTCATTATGAGAGGCAATGATT
GTTAATTTACAGCTTTCATGATTAGCAAGTTACAGTGATGCTGTGCTATGAATTTTCAAGTAATTGTGA
AAAAGTTAAACATTGAAGTAATGAATTTTTATGATATTCCCCCCACTTAAGACTGTGTATTCTAGTTTT
GTCAAACTGTAGAAATGATGATGTGGAAGAACTTAGGCATCTGTGGGCATGGTCAAAGGCTCAAACCTT
TATTTTAGAATTGATATACACGGATGACTTAACTGCATTTTTAGACCATTTATCTGGGATTATGGTTTT
GTGATGTTTGTCCTGAACACTTTTGTTGTAAAAAAATAATAATAATGTTTAATATTGAGAAAGAAACTA
ATATTTTATGTGAGAGAAAGTGTGAGCAAACTAACTTGACTTTTAAGGCTAAAACTTAACATTCATAGA
GGGGTGGAGTTTTAACTGTAAGGTGCTACAATGCCCCTGGATCTACCAGCATAAATATCTTCTGATTTG
TCCCTATGCATATCAGTTGAGCTTCATATACCAGCAATATATCTGAAGAGCTATTATATAAAAACCCCA
AACTGTTGATTATTAGCCAGGTAATGTGAATAAATTCTATAGGAACATATGAAAATACAACTTAAATAA
TAAACAGTGGAATATAAGGAAAGCAATAAATGAATGGGCTGAGCTGCCTGTAACTTGAGAGTAGATGGT
TTGAGCCTGAGCAGAGACATGACTCAGCCTGTTCCATGAAGGCAGAGCCATGGACCACGCAGGAAGGGC
CTACAGCCCATTTCTCCATACGCACTGGTATGTGTGGATGATGCTGCCAGGGCGCCATCGCCAAGTAAG
AAAGTGAAGCAAATCAGAAACTTGTGAAGTGGAAATGTTCTAAAGGTGGTGAGGCAATAAAAATCATAG
TACTCTTTGTAGCAAAATTCTTAAGTATGTTATTTTCTGTTGAAGTTTACAATCAAAGGAAAATAGTAA
TGTTTTATACTGTTTACTGAAAGAAAAAGACCTATGAGCACATAGGACTCTAGACGGCATCCAGCCGGA
GGCCAGAGCTGAGCCCTCAGCCCGGGAGGCAGGCTCCAGGCCTCAGCAGGTGCGGAGCCGTCACTGCAC
CAAGTCTCACTGGCTGTCAGTATGACATTTCACGGGAGATTTCTTGTTGCTCAAAAAATGAGCTCGCAT
TTGTCAATGACAGTTTCTTTTTTCTTACTAGACCTGTAACTTTTGTAAATACACATAGCATGTAATGGT
ATCTTAAAGTGTGTTTCTATGTGACAATTTTGTACAAATTTGTTATTTTCCATTTTTATTTCAAAATAT
ACATTCAAACTTAAAATTA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032991.3
Locus ID 836
MW 61.1
Summary The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. provided by RefSeq, Aug 2017
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Documents
Resources

file-alt Citations

Study of the molecular mechanisms by which non-coding RNAs (IncH19 and miR-675) control tumor progression and resistance to drug therapy in colorectal cancer
Zichittella
Thesis (2024)
Snippet: irVana Scrambled Negative Control (Scr) and with 50 ng of Reporter plasmid DNA (caspase-3 Human 3′ UTR Clone/RFP, Cat. n° SC215501, OriGene Technologies, Inc) used following the manufacturer’s standard application guide. Then 24 hours after transfection, luciferase tests were performed using the Firefly Luciferase Assay Kit (Cat
Mir-675-5p supports hypoxia-induced drug resistance in colorectal cancer cells
Zichittella, Barreca, Cordaro et al
BMC Cancer (2022) 22 (1), 567 DOI: 10.1186/s12885-022-09666-2
Cited by 23
AI Summary: In this study, the Caspase-3 Human 3′ UTR Clone/RFP plasmid (Cat. n° SC215501, OriGene Technologies, Inc) from OriGene was used in luciferase reporter assays to confirm Caspase-3 as a direct target of miR-675-5p, supporting the investigation of hypoxia-induced drug resistance mechanisms in colorectal cancer cells.
Snippet: mic miR-675-5p, Assay ID MC12067, Thermo Fisher , USA) or mirVana Scrambled Negative Control (Scr) and with 50 ng of Reporter plasmid DNA (Caspase-3 Human 3′ UTR Clone/RFP, Cat. n° SC215501, OriGene Technologies, Inc) used following the manufacturer’s standard application guide. Then 24 hours after transfection, luciferase tests were performed using the Firefly Luciferase Assay Kit (C
FigureFigureFigure
Showing 1-2 of 2 papers.
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