TJP1 (NM_003257) Human 3' UTR Clone

SKU SC214630

3' UTR clone of tight junction protein 1 (zona occludens 1) (TJP1) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: TJP1
• Synonyms: ZO-1
• ACCN: NM_003257
• Insert Size: 1477 bp
• Sequence Data:
  Insert Sequence

  >SC214630 3' UTR clone of NM_003257; The sequence shown below is from the reference sequence of NM_003257. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon ; ; ; CAATTGGCAGAGCTCAGAATTCAAGCGATCGC; ; TCTCGTTGGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAACTCTTGAAATATAGGAACTTAAAT; AATGTGAAACTGGATTAAACTTAATCTAAATGGAACCACTCTATCAAGTATTATACCTTTTTTAGAGTTG; ATACTACAGTTTGTTAGTATGAGGCATTTGTTTGAACTGATAAAGATGAGTGAGCATGCCCCTGAACCAT; GGTCGGAAAACATGCTACACACTGCATGTTTGTGATTGACGGGACTGTTGGTATTGGCTAGAGGTTCAAA; GATATTTTGCTTTGTGATTTTTGTAATTTTTTTATCGTCACTGCTTAACTTCACATATTGATTTCCGTTA; AAATACCAGCCAGTAAATGGGGGTGCATTTGAGGTCTGTTCTTTCCAAAGTACACTGTTTCAAACTTTAC; TATGGCCCTGGCCTAGCATACGTACACATTTTATTTTATTATGCATGAAGTAATATGCACACATTTTTTA; AATGCACCTGGAATATATAACCAGTGTTGTGGATTTAACAGAAATGTACAGCAAGGAGATTTACAACTGG; GGGAGGGTGAAGTGAAGACAATGACTTACTGTACATGAAAACACATTTTTCTTAGGGAAGGATACAAAAG; CATGTGAGACTGGTTCCATGGCCTCTTCAGATCTCTAACTTCACCATATTACCACAGACATACTAACCAG; CAGAAATGCCTTACCCTCATGTTCTTAATTCTTAGCTCATTCTCCTTGTGTTACTAAGTTTTTATGGCTT; TTGTGCATTATCTAGATACTGTATCATGACAAAGACTGAGTACGTTGTGCATTTGGTGGTTTCAGAAATG; TGTTATCACCCAGAAGAAAATAGTGGTGTGATTTGGGGATATTTTTTTCTTTTCTTTTCTTTTCTTTTTT; TTTTTTTTTGACAAGGGGCAGTGGTGGTTTTCTGTTCTTTCTGGCTATGCATTTGAAAATTTTGATGTTT; TAAGGATGCTTGTACATAATGCGTGCATACCACTTTTGTTCTTGGTTTGTAAATTAACTTTTATAAACTT; TACCTTTTTTATACATAAACAAGACCACGTTTCTAAAGGCTACCTTTGTATTCTCTCCTGTACCTCTTGA; GCCTTGAACTTTGACCTCTGCAGCAATAAAGCAGCGTTTCTATGACACATGCAAGGTCATTTTTTTTAAG; AAAAAGGATGCACAGAGTTGTTACATTTTTAAGTGCTGCATTTAAAAGATACAGTTACTCAGAATTCTCT; AGTTTGATTAAATTCTTGCAAAGTATCCCTACTGTAATTTGTGATACAATGCTGTGCCCTAAAGTGTATT; TTTTTACTAATAGACAATTTATTATGGCACATCAGCACGATTTCTGTTTAGATAATACACCACTACATTC; TGTTAATCATTAGGTGTGACTGAATTTCTTTTGCCGTTATTAAAAATCTCAAATTTCTAAATCTCCAAAA; TAAAACT; ; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003257.3
• Locus ID: 7082
• Summary: This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]