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HIF1A (NM_001530) Human 3' UTR Clone

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SKU
SC213108
3' UTR clone of hypoxia inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) transcript variant 1 for miRNA target validation
  $683.00
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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol HIF1A
Synonyms bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
ACCN NM_001530
Insert Size 1203 bp
Sequence Data
Insert Sequence
>SC213108 3’UTR clone of NM_001530
The sequence shown below is from the reference sequence of NM_001530. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
GCGATCGCCGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCTTTTTCTTAATTTCATTCC
TTTTTTTGGACACTGGTGGCTCATTACCTAAAGCAGTCTATTTATATTTTCTACATCTAATTTTAGAAG
CCTGGCTACAATACTGCACAAACTTGGTTAGTTCAATTTTGATCCCCTTTCTACTTAATTTACATTAAT
GCTCTTTTTTAGTATGTTCTTTAATGCTGGATCACAGACAGCTCATTTTCTCAGTTTTTTGGTATTTAA
ACCATTGCATTGCAGTAGCATCATTTTAAAAAATGCACCTTTTTATTTATTTATTTTTGGCTAGGGAGT
TTATCCCTTTTTCGAATTATTTTTAAGAAGATGCCAATATAATTTTTGTAAGAAGGCAGTAACCTTTCA
TCATGATCATAGGCAGTTGAAAAATTTTTACACCTTTTTTTTCACATTTTACATAAATAATAATGCTTT
GCCAGCAGTACGTGGTAGCCACAATTGCACAATATATTTTCTTAAAAAATACCAGCAGTTACTCATGGA
ATATATTCTGCGTTTATAAAACTAGTTTTTAAGAAGAAATTTTTTTTGGCCTATGAAATTGTTAAACCT
GGAACATGACATTGTTAATCATATAATAATGATTCTTAAATGCTGTATGGTTTATTATTTAAATGGGTA
AAGCCATTTACATAATATAGAAAGATATGCATATATCTAGAAGGTATGTGGCATTTATTTGGATAAAAT
TCTCAATTCAGAGAAATCATCTGATGTTTCTATAGTCACTTTGCCAGCTCAAAAGAAAACAATACCCTA
TGTAGTTGTGGAAGTTTATGCTAATATTGTGTAACTGATATTAAACCTAAATGTTCTGCCTACCCTGTT
GGTATAAAGATATTTTGAGCAGACTGTAAACAAGAAAAAAAAAATCATGCATTCTTAGCAAAATTGCCT
AGTATGTTAATTTGCTCAAAATACAATGTTTGATTTTATGCACTTTGTCGCTATTAACATCCTTTTTTT
CATGTAGATTTCAATAATTGAGTAATTTTAGAAGCATTATTTTAGGAATATATAGTTGTCACAGTAAAT
ATCTTGTTTTTTCTATGTACATTGTACAAATTTTTCATTCCTTTTGCTCTTTGTGGTTGGATCTAACAC
TAACTGTATTGTTTTGTTACATCAAATAAACATCTTCTGTGGACCAGGCACGCGTAAGCGGCCGCGGCA
TCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
Locus ID 3091
MW 47.4
Summary This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. provided by RefSeq, Jul 2011
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