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TIMM50 (NM_001001563) Human 3' UTR Clone

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SKU
SC212205
3' UTR clone of translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) (TIMM50) nuclear gene encoding mitochondrial protein for miRNA target validation
  $683.00
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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol TIMM50
Synonyms MGCA9; TIM50; TIM50L
ACCN NM_001001563
Insert Size 1106 bp
Sequence Data
Insert Sequence
>SC212205 3' UTR clone of NM_001001563
The sequence shown below is from the reference sequence of NM_001001563. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

CCTCACCAGCCGCTTGTGGCCTCGCTCCAAACAGCCCTGAACTCTGGGCCTCCTCAAACTCAGTGCCTGG
GTCCAGGGCCCCAGTGCTTCCAGACCAAGACTTGGGCCACCACTTGTCCAATAAAGTACATCCCAGACGC
CACACCTGCTGTGTCCCGAGAGTCTCCAGATGGGGGCATCAGGGTGAGGTCCGGGACTCTTGGGTCATCG
TCCCACAGTGGCTGATCGGCTGCCAAGCACAGTGGGGGTGCTTTGTTGGATCAGAGCAGATTTTTCACCC
TGGTCTCGGAATCTAAAAACCCTCGCTGTGTCTTCCTGTGTGTTGCGTGATCTGTGAAAAATACATCTCC
CTCTGACCACAGACTCCTCATCTGTGGAGAAGGGAAGAGAGGATGGCACTTGGCTTTAAAGACAGGTAGC
TGCCAGGCTACTGTGGAGAGAATGTGTTATGGGCCTAGTGGGCCTCTGGTGGACCAGAGATTACCCCTCT
CAACAGGCCAGCTGCTGGTTGGCTCCAGCCAGTGTGGCCCTGGGGTAAAGGGGGCCTGGAGTTGCTTGAC
ACGCCAGTTGTTCAAAAGAAGCAAGAACTTGGGAATATTTTTTTTTTTTTTTTGAGACAGGGTCTCACTG
TTGCCCAGGCTGGAGTGCATTGATGTGATCTTAGCTTACAACAGCCTTGACCTCCCGGCTCAAGTGATCT
CCAAGTGACCCTCCTGCCTCAGCCTCCTGAGTACCCTCCTGGGGACTACAGGTGCGTGCTACCATACCCA
GCTAATTTAAACAAATTTTTTTTTTGTAGAGATGGGGTCTTGGTGCCCAAGCTGGTCTCAAACTCCTAGT
CTTAAGCAATCTTCCTGCTTTGGCCTCCCAAGGCGCTGGGATTCCAGGTGTGAGCTACTGTGCCCAGCTA
AAATAGGGATTTTTATATTAAATCTCCCTATTTTAAAATGTTGGCTCCTAGCGGGGCGCAGTGGCTCACG
CCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATCGCCTGAGGTCAGGAGTTCAAGACCAGCCT
CAACATGGAGAAACCCCATCTCTGCTAAAAATACAAAATTAGCTGGGCGTGGTGGA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001001563.1
Locus ID 92609
Summary This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis. provided by RefSeq, Jul 2016
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