DNA PKcs (PRKDC) (NM_001081640) Human 3' UTR Clone

SKU SC212192

3' UTR clone of protein kinase DNA-activated catalytic polypeptide (PRKDC) transcript variant 2 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: DNA PKcs
• Synonyms: DNA-PKC; DNA-PKcs; DNAPK; DNAPKc; DNPK1; HYRC; HYRC1; IMD26; p350; XRCC7
• ACCN: NM_001081640
• Insert Size: 1092 bp
• Sequence Data:
  Insert Sequence

  >SC212192 3’UTR clone of NM_001081640 ; The sequence shown below is from the reference sequence of NM_001081640. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; ACCTGGGAAGGATGGGAGCCCTGGATGTGAGGTCTGTGGGAGTCTGCAGATAGAAAGCATTACATTGTT; TAAAGAATCTACTATACTTTGGTTGGCAGCATTCCATGAGCTGATTTTCCTGAAACACTAAAGAGAAAT; GTCTTTTGTGCTACAGTTTCGTAGCATGAGTTTAAATCAAGATTATGATGAGTAAATGTGTATGGGTTA; AATCAAAGATAAGGTTATAGTAACATCAAAGATTAGGTGAGGTTTATAGAAAGATAGATATCCAGGCTT; ACCAAAGTATTAAGTCAAGAATATAATATGTGATCAGCTTTCAAAGCATTTACAAGTGCTGCAAGTTAG; TGAAACAGCTGTCTCCGTAAATGGAGGAAATGTGGGGAAGCCTTGGAATGCCCTTCTGGTTCTGGCACA; TTGGAAAGCACACTCAGAAGGCTTCATCACCAAGATTTTGGGAGAGTAAAGCTAAGTATAGTTGATGTA; ACATTGTAGAAGCAGCATAGGAACAATAAGAACAATAGGTAAAGCTATAATTATGGCTTATATTTAGAA; ATGACTGCATTTGATATTTTAGGATATTTTTCTAGGTTTTTTCCTTTCATTTTATTCTCTTCTAGTTTT; GACATTTTATGATAGATTTGCTCTCTAGAAGGAAACGTCTTTATTTAGGAGGGCAAAAATTTTGGTCAT; AGCATTCACTTTTGCTATTCCAATCTACAACTGGAAGATACATAAAAGTGCTTTGCATTGAATTTGGGA; TAACTTCAAAAATCCCATGGTTGTTGTTAGGGATAGTACTAAGCATTTCAGTTCCAGGAGAATAAAAGA; AATTCCTATTTGAAATGAATTCCTCATTTGGAGGAAAAAAAGCATGCATTCTAGCACAACAAGATGAAA; TTATGGAATACAAAAGTGGCTCCTTCCCATGTGCAGTCCCTGTCCCCCCCCGCCAGTCCTCCACACCCA; AACTGTTTCTGATTGGCTTTTAGCTTTTTGTTGTTTTTTTTTTTCCTTCTAACACTTGTATTTGGAGGC; TCTTCTGTGATTTTGAGAAGTATACTCTTGAGTGTTTAATAAAGTTTTTTTCCAAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001081640.2
• Locus ID: 5591
• Summary: This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]