Beclin 1 (BECN1) (NM_003766) Human 3' UTR Clone

SKU SC208275

3' UTR clone of beclin 1 autophagy related (BECN1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Beclin 1
• Synonyms: ATG6; beclin1; VPS30
• ACCN: NM_003766
• Insert Size: 661 bp
• Sequence Data:
  Insert Sequence

  >SC208275 3' UTR clone of NM_003766; The sequence shown below is from the reference sequence of NM_003766. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon ; ; ; CAATTGGCAGAGCTCAGAATTCAAGCGATCGC; ; TTGCTTGGGTGTCCTCACAATTTTATAACAAATGACTTTTTTCCTTAGGGGGAGGTTTGCCTTAAAGGCT; TTTAATTTTGTTTTGTTTGCAAACATGTTTTAAATTAAATTCGGGTAATATTAAACAGTACATGTTTACA; ATACCAAAAAAGAAAAAATCCACAAAAGCCACTTTATTTTAAAATATCATGTGACAGATACTTTCCAGAG; CTACAACATGCCATCTATAGTTGCCAGCCCTGGTCAGTTTTGATTCTTAACCCCATGGACTCCTTTCCCT; TTCTTCTCTGAAAAAAACTAATTTAAATTTGCTTTTCTTTTTTTTAACTGAGTTGAATTGAGATTGATGT; GTTTTCACTGGATTTTTATCTCTCTCAACTTCCTGCACTTAACAATATGAAATAGAAACTTTTGTCTTTA; CTGAGATGAGGATATGTTTGAGATGCACAGTTGGATAATGTGGGAAAATGACATCTAAGCTTTACCTGGT; CACCATGTGATGTGATCAGATGCTTGAAATTTAACACTTTTCACTTGGTTCTTATACTGAATGCCGACTC; TGCTCTGTGTTAGAGATATGAAATGGTGTTTGATACTGTTTGAGACATTATGGAGAGATTTAATTATTTG; TAATAAAAGATTTGCTGCAGTCTGAAAACTG; ; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003766.3
• Locus ID: 8678
• Summary: This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]