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BRIT1 (MCPH1) (NM_024596) Human 3' UTR Clone

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SKU
SC207604
3' UTR clone of microcephalin 1 (MCPH1) for miRNA target validation
  $683.00
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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol BRIT1
Synonyms BRIT1; MCT
ACCN NM_024596
Insert Size 2000 bp
Sequence Data
Insert Sequence
>SC207604 3’UTR clone of NM_024596
The sequence shown below is from the reference sequence of NM_024596. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
GCCCCTGAAAACTACCTATTGTCACAATGACAGTGACCTCACTGGCCTGTGGTGACTGCACACAGCTCG
CAAAACTGTCTTTGGATGTTCAAATGAGAAACAAAACTGTGAAGAGAAGGAACTGGCGTATACAAGATG
ACTTCTGATATCATGTTTGCCATGTGTTGTGGTTCTTAAGAACTCATAGGTGACTTTCTGATGACTGAA
TGTCTGTTTCAGAGACGCTTCGGGCCTTTTTATTTTTATTTTATTTTTTATTTTTTGAGACGGAGTCCT
GCCCTGTTTCCCAGGCTGGAGTGCAATGGCACAATCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCA
AGCGATTCTGCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGATGTGTGCCACCATGCCTGGCTAATT
TTTGTAGTTTTAGTAGAGACAGGGTTTCGCCATGTTGGCCAGGCTGGTCTCAAACGCCTGAGCTCAGGT
GATCTGTCAGGCCTCTTCTATAGAATTCCAGTCTTTGTGTCTTAGTCATGATCATAATTGAAAGGTCAC
AGAACCTTTGTCATTAGAGCACAGTACTGCCAAATAAAGAATGGAAATTCAATGACATTGTTTTATTAC
TGAGAACAACTAGAGAACTCTGCAAGTTTCTTGGCTTAGACTCGATCTTTATTAATACATTATCTATTA
GGTAGGAAAGACATTTGTCAGCTATTAAGGTGACTTTTATCTAGCGGAGATTCCTCTCTTAAAGTAATG
AAAGGAGATAGGTATGGGGGGTGTTATACAGGATAATTGGTGACATCTGAGTGTCTTACTTCTGCAAGC
CTGCTTTATGGTGAGCAAAGCATCACCAGCAAGTGATCACAATGTCCACTGGCCGCTTTTTGCCTGCCG
TCCTCGAGATGAAATTGGCAGTTGGGGCTGATTCACAGAAACACCGATTTGTGGCTGAGCACGGTGGCT
CACACCTGTCATCCCAGCCCTTTGGGAGGCTGAGGTGGACAGATCACTTGAGGTCAGGAGTTCGAGACC
AGCCTGACCAACGCAGCAAAACCCATCTCTACTAAAAATACAAAAATCAGCTGGGTGTGGTGGCACACA
CCTGTGGTCCCAGCTCCTCAGGAGTCTGAGGCAGAAGAATCGCTTGAACCCAAGAGGCAGAGGTTGCAG
TGAGCCAAGGTTGCAGTGAATCAAGATTGCTCCACTGCACTCCAGCCTGGGCAACAGAGTAACTCTCCT
TCTCAAATAAATAAATAAATAAATAAGAAACACTGATGTGTCTGTCACCTTCTAAAGAAATGAAATGCT
AGGAAGTCCTAGCCAGAGTGATCAGGCAAGAATAAGCCATAAAAGGCATCCAAATAGGAAAAGAAGTCA
AACTGTCTCTCTTCACTGCCGATATGATTCTATACCTAGAAAACCCTAAAGACTCTGCCAAAAGGCTCC
TGGAACCGATAAATGACTTAAGTAAAGTTTCAGGATAGTAAATCCATGTACAAAAATCAGCATTTCCAA
ACACAGTAACATTCAAGCTGAGCACCAAATCAAGAACGCAATCCCATTTCCAATAGCCACGGAATGAAA
TACCTAGGAACACGTATAACCAAGGAGGCAAAGGATCTCTACAAGGAGAACCATAAACGAGATGCTGAG
TCCCAGCGAGGTCGGAGGTGCCACTGAGCCCTCATCGTGGTGCCGTTCCCGCTCTGGGTTATTTATCTG
TTGCTCATCTCAGCTGTTGTTCCTACCTCAAATTTCAAGTCCCTCAACAAATATAACAGAACCACTTCT
AGAATGAACCTTTGAGAAGGGAGGTAGCAGTGCATTGTATAGGAATTGGCATTCTATAGAAAACCACAG
AAACTGGAAATAATGAAGGGTTGTCTCTTGGTTTTAAAATAATGTATACACCTAAATCATCCCCTTATG
ATACTCATCCTCTAACAGCAATTGAACTTCAATACAATGAGTCATTCCTGAGTTCACTCGCTTCACAT
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024596.5
Locus ID 79648
MW 75.4
Summary This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. provided by RefSeq, Feb 2010
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