4 hours incubations; 30min washing and analyzing samples
Signal
Colorimetric
Curve Range
156pg/ml-10000pg/ml
Sample Type
Human serum, plasma and other biological fluids
Sample Volume
100µl
Specificity
This kit is used for quantitative detection of Human MAGEA4
Sensitivity
33.5pg/ml
Reactivity
Human
Cross Reactivity
Minimal cross-reactivity (about 6.6%) with recombinant human MAGEA4 protein. No significant cross-reactivity with other MAGEA family proteins.
Interference
No significant interference observed with available related molecules.
Components
MAGEA4 Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
Recombinant Human MAGEA4 Standard (500ng/ml) | 0.1 mL
100x Biotinylated Human MAGEA4 Detection Antibody | 0.12 mL
100x Peroxidase Conjugate | 0.12 mL
Assay Buffer | 30 mL
Standard Diluent | 10 mL
Sample Diluent1 | 15 mL
Sample Diluent2 | 15 mL
Wash Buffer Concentrate 20X | 60 mL
TMB Substrate | 12 mL
Stop Solution | 12 mL
Background
Human melanoma-associated antigen 4 (MAGEA4) is a protein encoded by MAGEA4 gene. This gene is a member of the MAGEA gene family. The members of this gene family encode proteins with 50 to 80% sequence identity to each other. MAGEA4 is highly expressed in a variety of cancers such as melanoma, bladder, breast, head and neck, oral, liver and lung cancers, but not in normal tissues except for testes and placenta. There is significant correlation between MAGEA4 protein expression and tumor invasion.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.