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Mouse Csf2 ELISA KIT (1 x 96 wells)

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SKU
EA102893
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Gm-Csf/CSF2 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Gm-Csf/CSF2 tissue specificity:
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse GM-CSF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Specificity This kit is used for quantitative detection of Mouse GM-CSF
Sensitivity <1pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • Description|Quantity
  • 96-well plate|1
  • lyophilized Standard
  • Biotinylated detection antibody (50x)|100 ul
  • Avidin-Biotin-Peroxidase Complex (30x)|400 µl
  • Sample diluent|30 ml
  • Antibody diluent|12 ml
  • Avidin-Biotin-Peroxidase Diluent|12 ml
  • Wash Buffer (25x)|20 ml
  • Color Developing Reagent (TMB)|10 ml
  • Stop Solution|10 ml
  • Plate Sealers|4
Background Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa.
Gene Symbol Csf2
Locus ID 12981
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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