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Dengue virus IgG ELISA Kit

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SKU
EA101064
Dengue virus IgG ELISA Kit, minimum order quantity: 12 kits
  $265.00
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Specifications
Specifications
Product Data
Species Human
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Sample Type Serum
Sample Volume 100 ul (final volume, diluted)
Specificity Natural and recombinant human Dengue virus IgG
Sensitivity The OriGene Dengue Virus IgG Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Dengue Virus in Serum.
Reactivity Human
Interference No significant interference observed with available related molecules.
Components See the product information for more details
Background The Dengue Virus IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Dengue-2 Virus antigen. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Dengue Virus-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Dengue Virus-specific IgG antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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