Adenovirus IgM ELISA Kit
$275.00
Please Inquire*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Format | 96-well strip plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Length | 3 hours |
| Signal | Colorimetric |
| Sample Type | Cell culture supernatant, serum, plasma |
| Sample Volume | 10 ul |
| Specificity | Natural and recombinant human Adenovirus IgM |
| Reactivity | Human |
| Interference | No significant interference observed with available related molecules. |
| Components | See the product information for more details |
| Background | The Adenovirus IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Specimen samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false results. Microtiter wells as a solid phase are coated with inactivated Adenovirus grade 2 antigen. Pretreated sample specimens and ready-for-use controls are pipetted into these wells. During incubation Adenovirus-specific antibodies of high-analyte specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of analyte presence) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Adenovirus-specific IgM antibody in the sample specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader. |
| Shipping | Blue Ice |
Reviews
Documents
| Datasheet | |
| SDS | |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
