Cortisol ELISA Kit

CAT#: EA101037

Cortisol ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0 - 800 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 20 ul
• Specificity: Natural and recombinant human Cortisol
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The ORIGENE Cortisol ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Cortisol in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Cortisol in the donor sample. The microtiter wells are coated with a monoclonal antibody directed towards an antigenic site on the Cortisol molecule. Endogenous Cortisol of a donor sample competes with a Cortisol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of cortisol in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of cortisol in the specimen sample.