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Cortisol ELISA Kit

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SKU
EA101037
Cortisol ELISA Kit
  $275.00
6 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 0 - 800 ng/mL
Sample Type Cell culture supernatant, serum, plasma
Sample Volume 20 ul
Specificity Natural and recombinant human Cortisol
Reactivity Human
Interference No significant interference observed with available related molecules.
Components See the product information for more details
Background The ORIGENE Cortisol ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Cortisol in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Cortisol in the donor sample. The microtiter wells are coated with a monoclonal antibody directed towards an antigenic site on the Cortisol molecule. Endogenous Cortisol of a donor sample competes with a Cortisol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of cortisol in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of cortisol in the specimen sample.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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