AFP ELISA Kit

SKU
EA101028
AFP ELISA Kit
  $260.00
6 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 0 - 160 IU/mL
Sample Type Cell culture supernatant, serum, plasma
Sample Volume 25 ul
Specificity Natural and recombinant human AFP
Reactivity Human
Interference No significant interference observed with available related molecules.
Components See the product information for more details
Background The AFP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal mouse antibody directed towards a unique antigenic site on an AFP molecule. An aliquot of specimen sample containing endogenous AFP is incubated in the coated well with enzyme conjugate, which is an anti-AFP antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of AFP in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of AFP in the specimen sample.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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