Human Secretory IgA ELISA kit

CAT#: EA100882

Human Secretory IgA ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 6.25-200 µg/dl
• Sample Type: Human stool or saliva
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human Secratory IgA
• Sensitivity: 6.25 µg/dl
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwell plate coated with anti-sIgA monoclonal Ab: 12x8x1
  • 2. sIgA Standard: 7 vials ( ready to use): 0.125ml
  • 3. Anti-sIgA Enzyme Conjugate: 1 vial (ready to use): 12ml
  • 2. sIgA Bi-level Control: 2 vials (ready to use): 0.125ml
  • 5. Incubation Buffer: 1 Bottle (Ready to use): 12ml
  • 6. sIgA Sample Diluent: 3 Bottles: 3 x 20ml
  • 7. TMB Substrate: 1 Bottle (ready to use): 12ml
  • 8. Stop Solution: 1 Bottle (ready to use): 12ml
  • 9. 20X Wash concentrate: 1 bottle: 25ml
• Background: The Secretory IgA (sIgA) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-sIgA monoclonal antibody, along with the incubation buffer. After a simple washing step, an anti-SIgA enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, a chromogenic substrate (TMB) is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of sIgA in the samples. A standard curve is generated relating color intensity to the concentration of sIgA.