Human C-Reactive Protein ELISA kit

CAT#: EA100881

Human C-Reactive Protein ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0.005-0.1 mg/l
• Sample Type: Serum
• Sample Volume: 5 µl/well
• Specificity: This kit is used for quantitative detection human C-Reactive Protein
• Sensitivity: 0.005 mg/l
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with CRP MAb: 12x8x1
  • 2. CRP Standard: 6 vials ( ready to use): 0.25ml
  • 3. CRP Enzyme Conjugate: 1 bottle (ready to use): 12 ml
  • 4. TMB Substrate: 1 bottle (ready to use): 12ml
  • 5. Stop Solution: 1 bottle (ready to use): 12ml
  • 6. Sample Diluent: 2 bottles (2x 25 ml): 50 ml
  • 7. 20X Wash concentrate: 1 bottle: 25ml
• Background: C-Reactive protein (CRP) is an alpha globulin with a molecular mass of approximately 110,000 to 140,000 daltons, and is composed of five identical subunits, which are noncovalently assembled as a cyclic pentamer. CRP is synthesized in the liver and is normally present as a trace constituent of serum or plasma at levels less than 0.3 mg/dl. CRP is one of the acute-phase proteins, the serum or plasma levels of which rise during general, nonspecific response to a wide variety of diseases. Although the detection of elevated levels of CRP in the serum is not specific for any particular disease, it is a useful indicator of inflammatory processes. Additionally, measurement of CRP by high-sensitivity CRP assays may add to the predictive value of other cardiac markers (myoglobin, creatine-kinase-MB, troponin I and T), which are used to assess the risk of cardiovascular and peripheral vascular disease. Inflammation in the arteries may play a role in heart disease and HS-CRP can determine heart disease risk in those with undetected heart disease and risk of complications for those who have already had a heart event.
• Gene Symbol: CRP
• Gene ID: 1401