Human Estradiol ELISA kit

CAT#: EA100858

Human Estradiol ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 10-1000 pg/ml
• Sample Type: Serum
• Sample Volume: 25 µl/well
• Specificity: This kit is used for quantitative detection human Estradiol
• Sensitivity: 8.7 pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with Steptavidin: 12x8x1
  • 2. Estradiol Standards: 6 vials (Ready to use): 0.5 ml
  • 3. Estradiol Biotin conjugate, 1 bottle (Ready to use): 7 ml
  • 4. Estradiol Enzyme conjugate concentrate, 20x, 1 Vial: 0.7 ml
  • 5. Assay Diluent , 1 bottle (Ready to use): 12ml
  • 6. TMB Reagent, 1 bottle (Ready to use): 12 ml
  • 7. Stop Solution , 1 bottle (Ready to use): 12 ml
  • 8. Wash Concentrate 20X: 1 bottle: 25 ml
• Background: Estradiol E2 is the most potent natural Estrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Estradiol is secreted into the blood stream where 98% bound to sex hormone binding globulin (SHBG). Estrogenic activity is effected via estradiol-receptor complexes which trigger the appropriate response at the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy. Serum Estradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls and primary and secondary amenorrhea and menopause. Estradiol levels have been reported to be increased in patients with feminizing syndromes, gynaecomastia and testicular tumors. In cases of infertility, serum Estradiol measurements are useful for monitoring induction of ovulation following treatment.