Human PCSK9 ELISA Kit

CAT#: EA100676

Human PCSK9 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human PCSK9
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10,000pg/ml
• Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human PCSK9
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human PCSK9 antibody | 1
  • Lyophilized recombinant human PCSK9 standard 10ng/tube | 2
  • Biotinylated anti- human PCSK9 antibody 130ul (dilution 1:100) | 1
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul (dilution 1:100) | 1
  • Sample diluent buffer 30ml | 1
  • Antibody diluent buffer 12ml | 1
  • ABC diluent buffer 12ml | 1
  • TMB color developing agent 10ml | 1
  • TMB stop solution 10ml | 1
  • Wash Buffer (25 x) 20ml | 1
  • Plate Sealers | 4
• Background: Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A(EGF-A) domain of the low-density lipoprotein receptor(LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins(LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
• Gene Symbol: PCSK9
• Gene ID: 255738