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Mouse IL15RA ELISA Kit

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SKU
EA100672
Mouse IL15RA ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse IL15RA
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 125pg/ml-8000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse IL15RA
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse IL15RA antibody 1
  • Lyophilized recombinant mouse IL15RA standard 10ng/tubex2
  • Biotinylated anti- mouse IL15RA antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.
Gene Symbol Il15ra
Locus ID 16169
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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