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Mouse MMP-12 ELISA Kit

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SKU
EA100640
Mouse MMP-12 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse MMP-12
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse MMP-12
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse MMP-12 antibody 1
  • Lyophilized recombinant mouse MMP-12 standard 10ng/tubex2
  • Biotinylated anti- mouse MMP-12 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Gene Symbol Mmp12
Locus ID 17381
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Matrix metalloproteinase 12 promotes tumor propagation in the lung
Ella, Harel, Abraham et al
J. Thorac. Cardiovasc. Surg. (2018)
Cited by 36
AI Summary: OriGene’s MMP-12 ELISA kit (EA100640) was used in this study to quantitatively measure matrix metalloproteinase 12 levels in tumor and normal lung samples, enabling the authors to assess the association between MMP-12 expression and lung tumor progression.
Snippet:   according to the manufacture's instructions (MMP-12 Elisa kit - EA100640 - by 277 OriGene Technologies - Rockville, MD 20850 USA) (for each of the samples (five 278 tumor and three normal lung) the ELISA was done in duplicates). Protein content in 279 
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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