Human IGFBP6 ELISA Kit

CAT#: EA100611

Human IGFBP6 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human IGFBP6
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human IGFBP6
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components: 96-well plate precoated with anti- human IGFBP6 antibody 1
  Lyophilized recombinant human IGFBP6 standard 10ng/tubex2
  Biotinylated anti- human IGFBP6 antibody 130ul(dilution 1:100)
  Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  Sample diluent buffer 30 ml
  Antibody diluent buffer 12ml
  ABC diluent buffer 12ml
  TMB color developing agent 10ml
  TMB stop solution 10ml
  Wash Buffer (25x) 20ml
  Plate Sealers 4x pieces
• Background: Insulin-like growth factor-binding protein 6(IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.
• Gene Symbol: IGFBP6
• Gene ID: 3489