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Human Endothelin ELISA Kit

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SKU
EA100599
Human Endothelin ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Endothelin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 3.9pg/ml-250pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Endothelin
Sensitivity <0.5pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-human EDN1 antibody | 1
  • Lyophilized recombinant human EDN1 standard | 1 ng/tube x 2
  • Biotinylated anti-human EDN1 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
Background The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.
Gene Symbol EDN1
Locus ID 1906
Shipping Blue Ice
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Documents
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file-alt Citations

Sebocytes contribute to melasma onset
Flori, Mastrofrancesco, Mosca et al
iScience (2022) 25 (3), 103871 DOI: 10.1016/j.isci.2022.103871
Cited by 43
AI Summary: OriGene’s SZ95 sebocyte cell line was used in this study to investigate how sebocytes contribute to melasma by analyzing their response to UVA radiation and their effects on pigmentation and inflammation.
FigureFigureFigure
Sebocytes contribute to melasma onset
Flori, Mastrofrancesco, Mosca et al
iScience (2022) 25 (3), 103871 DOI: 10.1016/j.isci.2022.103871
Cited by 43
AI Summary: In this study, OriGene products—specifically the Human SCF ELISA Kit (Cat#EA102189), Human Endothelin ELISA Kit (Cat#EA100599), and Human FGF1/FGF basic ELISA Kit (Cat#EA102179)—were used to quantify key pro-melanogenic and growth factors, helping to reveal how sebocytes contribute to melasma onset.
Snippet: Human IL-6 ELISA Kit Diaclone SAS Cat#950.030.192;2 x 96 (pre-coated) Human IL-8 ELISA Kit Diaclone SAS Cat#950.050.192; 2 x 96 (pre-coated) Human SCF ELISA Kit Origene Technologies Inc. Cat#EA102189 Human Endothelin ELISA Kit Origene Technologies Inc. Cat#EA100599 Human FGF1/FGF basic ELISA Kit Origene Technologies Inc. Cat#EA102179 Human AM
Showing 1-2 of 2 papers.
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